Bial's test

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Bial's test is a chemical test for the presence of pentoses. The components include orcinol, hydrochloric acid, and ferric chloride. A pentose, if present, will be dehydrated to form furfural which then reacts with the orcinol to generate a colored substance. The solution will turn bluish and a precipitate may form. The solution shows two absorption bands, one in the red between B and C and the other near the D line.[1] It is named after Manfred Bial, German Physician.


Bial's test.svg

Bial's reagent consists of 0.4g orcin, 200 ml of concentrated hydrochloric acid and 0.5 ml of a 10% solution of ferric chloride.[2] Bial's test is used to distinguish pentoses from hexoses; this distinction is based on the color that develops in the presence of orcinol and iron (III) chloride. Furfural from pentoses gives a blue or green color. The related hydroxymethylfurfural from hexoses may give a muddy-brown or gray solution, but this is easily distinguishable from the green color of pentoses.

Quantitative version[edit]

The test may be performed as a quantitative colorimetric test using a spectrophotometer. Fernell and King have published a procedure for simultaneous determination of pentoses and hexoses from measurements at two wavelengths [3] Various versions of this test are widely used for a quick chemical determination of RNA; in this context it is usually called the orcinol test.


  1. ^ Baldwin, E. and Bell, D.J., Cole's Practical Physiological Chemistry, published by Heffer, Cambridge, 1955, page 189
  2. ^ Baldwin and Bell, page 189
  3. ^ W. R. Fernell and H. K. King, The simultaneous determination of pentose and hexose in mixtures of sugars. Analyst, 1953,78, 80-83