File:FlowCitometer.svg
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Summary
| Description |
English: A source of monochromatic light, usually a laser beam (A) sends a beam that is collimated (L1) and focused (L2) by lenses on the laminar flow chamber (C), after passing through the camera, the direct light beam is arrested by a screen (s), while the scattered light is focused by another lens (L3) on a photodiode (D1), this constitutes the Forward SCatter detector FSC.
The side scattered light and the fluorescence focused by a lens onto a dichroic mirror (M) that reflects most of the light of wavelength equal to that produced by the source (A), goes through a filter (F1) and impinges on a photodiode detector (D2). This constitutes the Side SCatter Detector (SSC). The light that is not reflected by the dichroic mirror and which has a wavelength different from that emitted and impinges on it goes through an interference filter (F2) which can be adjusted to a specific wavelength, thus discriminating between different fluorophores , to ultimately impinge on a photomultiplier tube (D3), this is the Side FLuorescence detector (SFL)Español: Un emisor de luz monocromática, usualmente un haz de luz láser (A) envía un rayo que es colimado (L1) y enfocado (L2) sobre la cámara de flujo laminar (C), luego de atravesar la cámara el rayo de luz directa es detenido por una pantalla (s), mientras que la luz dispersada es enfocada por otra lente (L3) sobre un fotodiodo (D1), este constituye el detector de dispersión frontal (FSC).
La luz dispersada lateralmente y la fluorescencia se enfocan por medio de una lente sobre un espejo dicroico (M) que refleja la mayor parte de la luz de igual longitud de onda a la producida por la fuente (A), atravieza un filtro (F1) e incide sobre un detector (D2). Esto constituye el sistema de detección de dispersión lateral (SSC). La luz que no es reflejada por el espejo dicroico, ya que tiene una longitud de onda diferente a la emitida, lo atravieza e incide sobre un filtro interferencial (F2) que puede ser ajustado para una determinada longitud de onda, discriminando así entre diferentes fluoróforos, para finalmente incidir sobre un tubo fotomultiplicador (D3), esto constituye el detector lateral de fluorescencia (SFL). |
| Date | |
| Source | Own work |
| Author | J3D3 |
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 22:04, 18 August 2012 | | 607 × 380 (19 KB) | J3D3 |
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