Seliwanoff’s test is a chemical test which distinguishes between aldose and ketose sugars. Ketoses are distinguished from aldoses via their ketone/aldehyde functionality. If the sugar contains a ketone group, it is a ketose and if it contains an aldehyde group, it is an aldose. This test is based on the fact that, when heated, ketoses are more rapidly dehydrated than aldoses. It is named after Theodor Seliwanoff, the chemist that first devised the test.
- The acid hydrolysis of polysaccharides and oligosaccharides yields simpler sugars followed by furfural.
- The dehydrated ketose then reacts with the resorcinol to produce a deep cherry red color. Aldoses may react slightly to produce a faint pink color.
Fructose and sucrose are two common sugars which give a positive test. Sucrose gives a positive test as it is a disaccharide consisting of fructose and glucose.
- Abramoff, Peter; Thomson, Robert (1966). An experimental approach to biology. WH Freeman & Company, San Francisco. p. 47.
- Seliwanoff, Theodor (1887). "Notiz über eine Fruchtzuckerreaction". Berichte der deutschen chemischen Gesellschaft 20: 181. doi:10.1002/cber.18870200144.
- Katoch, Rajan (2011-06-30). Analytical Techniques in Biochemistry and Molecular Biology. p. 71. ISBN 978-1-4419-9784-5.
- Chawla (2003-01-01). Practical Clinical Biochemistry: Methods and Interpretations. p. 35. ISBN 978-81-8061-108-7.
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