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{{Infobox scientist
{{AfC submission|t||ts=20220725175418|u=Saritfantam|ns=118|demo=}}
| name = David W. Wood
| image = <!--(filename only, i.e. without "File:" prefix)-->
| caption = https://www.worldcat.org/title/generation-and-application-of-a-self-cleaving-protein-linker-for-use-in-single-step-affinity-fusion-based-protein-purification/oclc/1150068137&referer=brief_results
| workplaces = [[Ohio State University]], [[Princeton University]], [[Amgen]], [[Bristol Myers Squibb]], CP Kelco (former division of Merck & Co.)
| thesis_title = Generation and application of a self-cleaving protein linker for use in single-step affinity fusion based protein purification
| thesis_url = https://www.worldcat.org/title/generation-and-application-of-a-self-cleaving-protein-linker-for-use-in-single-step-affinity-fusion-based-protein-purification/oclc/1150068137&referer=brief_results
| thesis_year = 2000
| known_for = Self-removing affinity tag methods and protein engineering
| awards = NSF Career Award (2003),
Lumley Engineering Research Award, College of Engineering at Ohio State University (2016)
| website = https://cbe.osu.edu/wood-laboratory-applied-protein-engineering
| education = Rensselaer Polytechnic Institute (M.S. & Ph.D.),
California Institute of Technology (Caltech, B.S.)
| doctoral_advisors = Dr. Georges Belfort, Rensselaer Polytechnic Institute, Troy, NY, and Dr. [[Marlene Belfort]], [[Wadsworth Center]], New York State Dept. of Health, Albany, NY
}}


⚫
''David W. Wood'', Ph.D. (born in 1967) is an American chemical engineer, scientist, entrepreneur, inventor, and Professor of Chemical and Biomolecular Engineering at Ohio State University. Dr. Wood is also associated with the Department of Chemistry and Biochemistry and Molecular Biophysics Training Program.
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⚫
'''David W. Wood''', Ph.D. (born on March 15, 1967, in El Paso, Texas) is an American chemical engineer, scientist, entrepreneur and inventor. He is also Professor of Chemical and Biomolecular Engineering at the Ohio State University.
Dr. Wood is best known for his work on self-removing affinity tag methods, which he first published in Nature Biotechnology<ref name=":1">{{Cite journal |last=Wood |first=David W. |last2=Wu |first2=Wei |last3=Belfort |first3=Georges |last4=Derbyshire |first4=Victoria |last5=Belfort |first5=Marlene |date=1999 |title=A genetic system yields self-cleaving inteins for bioseparations |url=https://www.nature.com/articles/nbt0999_889 |journal=Nature Biotechnology |language=en |volume=17 |issue=9 |pages=889–892 |doi=10.1038/12879 |issn=1546-1696}}</ref> while a Ph.D. student at Rensselaer Polytechnic Institute. This innovative method was also patented as a part of a collaboration with extraordinary co-inventors at the Wadsworth Center of the New York State Department of Health and Rensselaer Polytechnic Institute, including Dr. [[Marlene Belfort]], Dr. Georges Belfort, Dr. Victoria Derbyshire, and Dr. Wei Wu.<ref>{{Cite patent|number=6933362|title=United States Patent: 6933362 - GENETIC SYSTEM AND SELF-CLEAVING INTEINS DERIVED THEREFROM, BIOSEPARATIONS AND PROTEIN PURIFICATION EMPLOYING SAME, AND METHODS FOR DETERMINING CRITICAL, GENERALIZABLE AMINO ACID RESIDUES FOR VARYING INTEIN ACTIVITY|gdate=August 23, 2005|invent1=Belfort|invent2=Belfort|invent3=Derbyshire|invent4=Wood|inventor1-first=Marlene|inventor2-first=Georges|inventor3-first=Vicky|inventor4-first=David|url=https://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO2&Sect2=HITOFF&p=1&u=/netahtml/PTO/search-bool.html&r=3&f=G&l=50&co1=AND&d=PTXT&s1=%22Self-Cleaving+Inteins%22&s2=wood.INNM.&OS=%22Self-Cleaving+Inteins%22+AND+IN/wood&RS=%22Self-Cleaving+Inteins%22+AND+IN/wood}}</ref>


== Background and education ==
== Background and education ==


⚫
Wood received dual undergraduate degrees in Biology and Chemical Engineering from the [[California Institute of Technology]] in 1990. He worked as an undergraduate in the lab led by Dr. [[Frances H. Arnold]]. He earned his Ph.D. in chemical engineering from [[Rensselaer Polytechnic Institute]] in 2000. His doctoral co-advisors were Dr. Georges Belfort at Rensselaer Polytechnic Institute, Troy, NY, and [[Marlene Belfort|Dr. Marlene Belfort]] at [[Wadsworth Center]], New York State Department of Health, Albany, NY.
Wood was born on March 15, 1967, in El Paso, TX, US.


⚫
== Career ==
⚫
He received his Biology and Chemical Engineering [[bachelor's degree]] from the California Institute of Technology in 1990. He earned his Ph.D. in chemical engineering from Rensselaer Polytechnic Institute in 2000 (Co-Advisors: Georges Belfort, Rensselaer Polytechnic Institute, Troy, NY, and Marlene Belfort, Wadsworth Center, New York State Dept. of Health, Albany, NY)


After finishing undergraduate dual degrees in biology and chemical engineering at Caltech in 1990, Wood started working on high-viscosity mucopolysaccharide fermentation development at [https://www.cpkelco.com/ CP Kelco] located on the San Diego Bay in San Diego, California. Soon after, he joined [[Amgen]] in 1991, the same year that Amgen received FDA approval for Neupogen®. This recombinant protein cytokine drug induces white blood cell production to fight infections in immunocompromised cancer patients after undergoing chemotherapy or radiation treatment.<ref name=":2">{{Cite web |title=Amgen History |url=https://www.amgen.com/about/amgen-history |access-date=2022-07-27 |website=Amgen, Inc |language=en}}</ref> Wood worked on the GMP fermentation team to manufacture [[Filgrastim|Neupogen]]® ([[Granulocyte colony-stimulating factor|rhG-CSF]]) between 1991-1993. As it is known today, Neupogen® became one of the most successful biotech drugs at that time and second blockbuster for Amgen after [[Epoetin alfa|Epogen]]®. In 1993, Wood joined the group of Dr. Georges Belfort<ref>{{Cite web |title=Georges Belfort {{!}} The Howard P. Isermann Department of Chemical and Biological Engineering |url=https://cbe.rpi.edu/people/faculty/georges-belfort |access-date=2022-07-27 |website=cbe.rpi.edu}}</ref> as a Ph.D. student at [[Rensselaer Polytechnic Institute]], where he worked primarily on mini-intein development for protein purification. During this time, he engineered the ∆I-CM intein, which was derived from ''[[Mycobacterium tuberculosis|Mycobacterium tuberculosis recA]]'' [[intein]].<ref name=":1" /><ref>{{Cite journal |last=Derbyshire |first=Victoria |last2=Wood |first2=David W. |last3=Wu |first3=Wei |last4=Dansereau |first4=John T. |last5=Dalgaard |first5=Jacob Z. |last6=Belfort |first6=Marlene |date=1997-10-14 |title=Genetic definition of a protein-splicing domain: Functional mini-inteins support structure predictions and a model for intein evolution |url=https://pnas.org/doi/full/10.1073/pnas.94.21.11466 |journal=Proceedings of the National Academy of Sciences |language=en |volume=94 |issue=21 |pages=11466–11471 |doi=10.1073/pnas.94.21.11466 |issn=0027-8424 |pmc=PMC23508 |pmid=9326633}}</ref><ref>{{Cite journal |last=Wood |first=D.W. |last2=Derbyshire |first2=V. |last3=Wu |first3=W. |last4=Chartrain |first4=M. |last5=Belfort |first5=M. |last6=Belfort |first6=G. |date=2000-12-01 |title=Optimized Single-Step Affinity Purification with a Self-Cleaving Intein Applied to Human Acidic Fibroblast Growth Factor |url=http://doi.wiley.com/10.1021/bp0000858 |journal=Biotechnology Progress |language=en |volume=16 |issue=6 |pages=1055–1063 |doi=10.1021/bp0000858 |issn=8756-7938}}</ref><ref>{{Cite web |title=Generation and application of a self -cleaving protein linker for use in single-step affinity fusion based protein purification - ProQuest |url=https://www.proquest.com/openview/3bb6a1e72ecf69b596f32c87ed1518f6/1?pq-origsite=gscholar&cbl=18750&diss=y |access-date=2022-07-27 |website=www.proquest.com |language=en}}</ref> After earning his Ph.D. he joined as a postdoctorate a team at [[Bristol Myers Squibb]] in Hopewell, New Jersey. This time he focused on the recovery and ''in vitro'' processing of transgenic monoclonal antibody therapeutics. In 2001, Dr. Wood started his academic career at [[Princeton University]] as an Assistant Professor of Chemical and Biological Engineering, where he continued his research focusing on self-removing tags, protein engineering, and applied biosensors.<ref>{{Cite journal |last=Wu |first=Wan-Yi |last2=Mee |first2=Courtney |last3=Califano |first3=Filomena |last4=Banki |first4=Reza |last5=Wood |first5=David W. |date=2006 |title=Recombinant protein purification by self-cleaving aggregation tag |url=https://www.nature.com/articles/nprot.2006.314 |journal=Nature Protocols |language=en |volume=1 |issue=5 |pages=2257–2262 |doi=10.1038/nprot.2006.314 |issn=1750-2799}}</ref><ref>{{Cite journal |last=Skretas |first=G. |date=2005-02-01 |title=Regulation of protein activity with small-molecule-controlled inteins |url=http://doi.wiley.com/10.1110/ps.04996905 |journal=Protein Science |language=en |volume=14 |issue=2 |pages=523–532 |doi=10.1110/ps.04996905 |issn=0961-8368 |pmc=PMC2386410 |pmid=15632292}}</ref><ref>{{Cite journal |last=Skretas |first=Georgios |last2=Meligova |first2=Aggeliki K. |last3=Villalonga-Barber |first3=Carolina |last4=Mitsiou |first4=Dimitra J. |last5=Alexis |first5=Michael N. |last6=Micha-Screttas |first6=Maria |last7=Steele |first7=Barry R. |last8=Screttas |first8=Constantinos G. |last9=Wood |first9=David W. |date=2007-07-01 |title=Engineered Chimeric Enzymes as Tools for Drug Discovery: Generating Reliable Bacterial Screens for the Detection, Discovery, and Assessment of Estrogen Receptor Modulators |url=https://pubs.acs.org/doi/10.1021/ja067754j |journal=Journal of the American Chemical Society |language=en |volume=129 |issue=27 |pages=8443–8457 |doi=10.1021/ja067754j |issn=0002-7863}}</ref><ref>{{Cite journal |last=Hartman |first=Izabela |last2=Gillies |first2=Alison R. |last3=Arora |first3=Sonia |last4=Andaya |first4=Christina |last5=Royapet |first5=Nitya |last6=Welsh |first6=William J. |last7=Wood |first7=David W. |last8=Zauhar |first8=Randy J. |date=2009-10-01 |title=Application of Screening Methods, Shape Signatures and Engineered Biosensors in Early Drug Discovery Process |url=https://doi.org/10.1007/s11095-009-9941-z |journal=Pharmaceutical Research |language=en |volume=26 |issue=10 |pages=2247–2258 |doi=10.1007/s11095-009-9941-z |issn=1573-904X}}</ref><ref>{{Cite journal |last=Li |first=Jingjing |last2=Gierach |first2=Izabela |last3=Gillies |first3=Alison R. |last4=Warden |first4=Charles D. |last5=Wood |first5=David W. |date=2011-11-15 |title=Engineering and optimization of an allosteric biosensor protein for peroxisome proliferator-activated receptor γ ligands |url=https://www.sciencedirect.com/science/article/pii/S0956566311005306 |journal=Biosensors and Bioelectronics |language=en |volume=29 |issue=1 |pages=132–139 |doi=10.1016/j.bios.2011.08.006 |issn=0956-5663}}</ref> In 2009, he joined Chemical and Biomolecular Engineering Department at [[Ohio State University]] as an Associate Professor and soon after was appointed as a Full Professor. While at the Ohio State University he continued expanding his research on self-removing tags to explore additional applications, including the development of multitarget sRNAs that can be used for metabolic engineering, and the modification of human butyrylcholinesterase for the degradation of the chemical warfare nerve agents in collaboration with the [[Battelle Memorial Institute]].<ref>{{Cite journal |last=Lahiry |first=Ashwin |last2=Stimple |first2=Samuel D. |last3=Wood |first3=David W. |last4=Lease |first4=Richard A. |date=2017-04-21 |title=Retargeting a Dual-Acting sRNA for Multiple mRNA Transcript Regulation |url=https://pubs.acs.org/doi/10.1021/acssynbio.6b00261 |journal=ACS Synthetic Biology |language=en |volume=6 |issue=4 |pages=648–658 |doi=10.1021/acssynbio.6b00261 |issn=2161-5063}}</ref><ref>{{Cite journal |last=McGarry |first=Kevin G |last2=Lalisse |first2=Remy F |last3=Moyer |first3=Robert A |last4=Johnson |first4=Kristyn M |last5=Tallan |first5=Alexi M |last6=Winters |first6=Tyson P |last7=Taris |first7=Joeseph E |last8=McElroy |first8=Craig A |last9=Lemmon |first9=Erin E |last10=Shafaat |first10=Hannah S |last11=Fan |first11=Yamin |date=2020-03-01 |title=A Novel, Modified Human Butyrylcholinesterase Catalytically Degrades the Chemical Warfare Nerve Agent, Sarin |url=https://doi.org/10.1093/toxsci/kfz251 |journal=Toxicological Sciences |volume=174 |issue=1 |pages=133–146 |doi=10.1093/toxsci/kfz251 |issn=1096-6080}}</ref> Currently, he has joint appointments with Department of Chemistry and Biochemistry and the Molecular Biophysics Training Program at the Ohio State University. He is one of a small group of researches worldwide focusing on intein implementation in various applications, along with [[Marlene Belfort|Dr. Marlene Belfort]] and Dr. Tom Muir.<ref>{{Cite journal |last=Prabhala |first=Sai Vivek |last2=Gierach |first2=Izabela |last3=Wood |first3=David W. |date=2022 |title=The Evolution of Intein-Based Affinity Methods as Reflected in 30 years of Patent History |url=https://www.frontiersin.org/articles/10.3389/fmolb.2022.857566 |journal=Frontiers in Molecular Biosciences |volume=9 |doi=10.3389/fmolb.2022.857566/full |issn=2296-889X}}</ref><ref>{{Citation |last=Wood |first=David W. |title=Industrial Applications of Intein Technology |date=2005 |url=https://doi.org/10.1007/3-540-29474-0_20 |work=Homing Endonucleases and Inteins |pages=345–364 |editor-last=Belfort |editor-first=Marlene |place=Berlin, Heidelberg |publisher=Springer |language=en |doi=10.1007/3-540-29474-0_20 |isbn=978-3-540-29474-0 |access-date=2022-07-27 |last2=Harcum |first2=Sarah W. |last3=Belfort |first3=Georges |editor2-last=Wood |editor2-first=David W. |editor3-last=Stoddard |editor3-first=Barry L. |editor4-last=Derbyshire |editor4-first=Victoria}}</ref>
⚫
==Career==
Wood is also associated with the Department of Chemistry and Biochemistry and Molecular Biophysics Training Program at Ohio State University. He was formerly Assistant Professor of Chemical and Biological Engineering at Princeton University between 2001 and 2009.


Wood is an author of over 60 publications and six issued patents, and two additional patent applications pending. His publications were cited nearly 4000 times.


Dr. Wood's research focuses on developing new technologies by recombining protein domains, particularly in biopharmaceutical development and manufacturing. Over the past several years, he has continued refining these methods for biopharmaceutical development and manufacturing, and his work has drawn funding from the DARPA BioMOD project (in collaboration with Dr. Govind Rao and Dr. Doug Frey Labs at UMBC), NIH, NSF and US Army Research Office, projects as well as several industry sponsors.<ref>{{Cite web |title=NSF Award Search: Award # 1264322 - Highly Controllable Self-cleaving Tags for Biopharmaceutical Research and Manufacturing Purification Platforms |url=https://www.nsf.gov/awardsearch/showAward?AWD_ID=1264322&HistoricalAwards=false |access-date=2022-07-27 |website=www.nsf.gov |language=en}}</ref><ref>{{Cite web |title=NSF Award Search: Award # 1264322 - Highly Controllable Self-cleaving Tags for Biopharmaceutical Research and Manufacturing Purification Platforms |url=https://www.nsf.gov/awardsearch/showAward?AWD_ID=1264322&HistoricalAwards=false |access-date=2022-07-27 |website=www.nsf.gov |language=en}}</ref><ref>{{Cite web |date=2015-07-28 |title=Just-in-time therapeutics manufacturing project receives additional DARPA funding |url=https://engineering.osu.edu/news/2015/07/just-time-therapeutics-manufacturing-project-receives-additional-darpa-funding |access-date=2022-07-27 |website=COLLEGE OF ENGINEERING |language=en}}</ref><ref>{{Cite web |title=Graduate students in the Frey Laboratory at UMBC |url=https://userpages.umbc.edu/~dfrey1/Freystud.html |access-date=2022-07-27 |website=userpages.umbc.edu}}</ref><ref>{{Cite web |date=2013-07-03 |title=Ohio State engineering faculty attract $3.3 million in NSF funding in June |url=https://engineering.osu.edu/news/2013/07/ohio-state-engineering-faculty-attract-3.3-million-nsf-funding-june |access-date=2022-07-27 |website=COLLEGE OF ENGINEERING |language=en}}</ref><ref>{{Cite web |date=2021-02-22 |title=Advancing the science and engineering of artificial blood |url=https://cbe.osu.edu/news/2021/02/advancing-science-and-engineering-artificial-blood |access-date=2022-07-27 |website=Chemical and Biomolecular Engineering |language=en}}</ref><ref>{{Cite journal |last=Banki |first=Mahmoud Reza |last2=Feng |first2=Liang |last3=Wood |first3=David W. |date=2005 |title=Simple bioseparations using self-cleaving elastin-like polypeptide tags |url=https://www.nature.com/articles/nmeth787 |journal=Nature Methods |language=en |volume=2 |issue=9 |pages=659–662 |doi=10.1038/nmeth787 |issn=1548-7105}}</ref> Dr. Wood was also involved in the development of protein switches for biotechnology funded by the NSF Career Award.<ref name=":0">{{Cite web |title=NSF Award Search: Award # 0348220 - CAREER: Protein Switches for Molecular Biotechnology |url=https://www.nsf.gov/awardsearch/showAward?AWD_ID=0348220&HistoricalAwards=false |access-date=2022-07-27 |website=www.nsf.gov |language=en}}</ref>
Wood is known for the development of inteins for various applications in bioseparation. He was also involved in protein engineering for biosensor development. Previously, he worked on GMP manufacturing of Neupogen® at Amgen (Thousand Oaks, CA) and high-viscosity mucopolysaccharide fermentation development at CP Kelco (San Diego, CA) of Merck & Co., at that time. While at Bristol-Myers Squibb (Hopewell, NJ) he was also working on the recovery and in vitro processing of transgenic monoclonal antibody therapeutics.

Dr. Wood is an author of over 60 publications, six issued patents, two additional patent applications pending and an additional dozen book chapters or edited volumes. His publications have been cited nearly 4000 times.<ref>{{Cite web |title=David W. Wood |url=https://scholar.google.com/citations?user=-MSZkqoAAAAJ&hl=en |access-date=2022-07-27 |website=scholar.google.com}}</ref>


Since Dr. Wood's research intersects with chemical engineering, molecular biology, biopharmaceutical development and manufacturing, his former undergraduate and graduate students have mostly entered industry positions at the top companies, which include Amgen, Genentech, Novartis, Biogen, Regeneron, Merck, WuXi Biologics, Shire Pharmaceuticals, Proctor & Gamble, Genzyme, GlaxoSmithKline, EirGenix, and Pfizer. Other former students have become professors at universities that include UT Austin, UC Irvine, Bucknell University, Smith College, The George Washington University, North Carolina State University, and the NYU School of Medicine. During the interview about careers in bioprocessing field for Science Magazine, he said "My primary advice to anyone considering this field is to take as many courses in engineering and biology as possible".<ref>{{Cite web |title=Melding talents with a career in bioprocessing |url=https://www.science.org/content/article/melding-talents-career-bioprocessing-0 |access-date=2022-07-27 |website=www.science.org |language=en}}</ref>


Wood is a member of the American Chemical Society, BIOT division, American Institute of Chemical Engineers, and International Society of Pharmaceutical Engineers.
Wood is a member of the American Chemical Society, BIOT division, American Institute of Chemical Engineers, and International Society of Pharmaceutical Engineers.
Line 25: Line 41:
=== Awards and honors ===
=== Awards and honors ===


* 2003: NSF Career Award
* 2003: NSF Career Award<ref name=":0" />
* 2016: Lumley Engineering Research Award, Ohio State College of Engineering
* 2016: Lumley Engineering Research Award, Ohio State College of Engineering<ref>{{Cite web |date=2016-04-18 |title=David Wood's research recognized with Lumley Award |url=https://cbe.osu.edu/news/2016/04/david-woods-research-recognized-lumley-award |access-date=2022-07-27 |website=Chemical and Biomolecular Engineering |language=en}}</ref>


=== Founder of Protein Capture Science ===
=== Founder of Protein Capture Science ===
Protein Capture Science<ref>{{Cite web |title=Protein Capture Science |url=https://www.proteincapturescience.com/ |access-date=2022-07-27 |website=www.proteincapturescience.com |language=en}}</ref> is a biotech start-up company commercializing a self-removing affinity tag technology for research and large-scale purifications of proteins.<ref>{{Cite web |date=2021-05-20 |title=Protein Capture Science Seeks to Accelerate Biomedical Research |url=https://www.rev1ventures.com/blog/protein-capture-science-seeks-to-accelerate-biomedical-research/ |access-date=2022-07-27 |website=Rev1 Ventures |language=en-US}}</ref><ref>{{Cite web |date=2022-04-15 |title=Protein Capture Science Accelerates Medical Therapies |url=https://www.rev1ventures.com/blog/protein-capture-science-accelerates-medical-therapies/ |access-date=2022-07-27 |website=Rev1 Ventures |language=en-US}}</ref> Dr. Wood and Dr. Izabela Gierach are co-founders of the company, based in Columbus, Ohio. The first line of the ''i''CapTag™ products for protein purification was showcased in the Industry Innovators 2021-2022 Issue of the journal BioProcess International.<ref>{{Cite web |date=2021-08-25 |title=iCapTag Tagless Protein Purification Platform |url=https://bioprocessintl.com/sponsored-content/the-icaptag-protein-purification-platform-bridging-research-and-manufacturing/ |access-date=2022-07-27 |website=BioProcess International |language=en-US}}</ref> Protein Capture Science was recently awarded several grants and funding, including highly competitive The Ohio Third Frontier Technology Validation and Start-Up Fund and the Concept Fund for extraordinary technologies by the Department of Development, State of Ohio.<ref>Ohio Advancing Cutting-Edge Technology, Third Frontier Accelerating New Ideas and Solutions.https://development.ohio.gov/static/business/thirdfrontier/2020%2006%2024%20Ohio%20Advancing%20Cutting-Edge%20Technology.pdf. Retrieved 2022-07-27</ref>
Protein Capture Science is a start-up company commercializing a self-cleaving affinity tag technology. Wood and Izabela Gierach are founders of the company, based in Columbus, Ohio.

{{Drafts moved from mainspace|date=July 2022}}
== External Links ==

* [https://www.youtube.com/watch?v=73wt-q4_lkU Izabela Gierach and David Wood - Protein Capture Science]
* [https://www.genscript.com/webinars/a-general-capture-purification-platform-for-tagless-proteins-based-on-a-self-cleaving-split-intein-tag.html A general capture and purification platform for tagless proteins based on a self-cleaving split-intein tag]

== References ==
<references />

Revision as of 19:48, 1 August 2022

David W. Wood
EducationRensselaer Polytechnic Institute (M.S. & Ph.D.), California Institute of Technology (Caltech, B.S.)
Known forSelf-removing affinity tag methods and protein engineering
AwardsNSF Career Award (2003), Lumley Engineering Research Award, College of Engineering at Ohio State University (2016)
Scientific career
InstitutionsOhio State University, Princeton University, Amgen, Bristol Myers Squibb, CP Kelco (former division of Merck & Co.)
ThesisGeneration and application of a self-cleaving protein linker for use in single-step affinity fusion based protein purification (2000)
Doctoral advisorsDr. Georges Belfort, Rensselaer Polytechnic Institute, Troy, NY, and Dr. Marlene Belfort, Wadsworth Center, New York State Dept. of Health, Albany, NY
Websitehttps://cbe.osu.edu/wood-laboratory-applied-protein-engineering

David W. Wood, Ph.D. (born in 1967) is an American chemical engineer, scientist, entrepreneur, inventor, and Professor of Chemical and Biomolecular Engineering at Ohio State University. Dr. Wood is also associated with the Department of Chemistry and Biochemistry and Molecular Biophysics Training Program.


Dr. Wood is best known for his work on self-removing affinity tag methods, which he first published in Nature Biotechnology[1] while a Ph.D. student at Rensselaer Polytechnic Institute. This innovative method was also patented as a part of a collaboration with extraordinary co-inventors at the Wadsworth Center of the New York State Department of Health and Rensselaer Polytechnic Institute, including Dr. Marlene Belfort, Dr. Georges Belfort, Dr. Victoria Derbyshire, and Dr. Wei Wu.[2]

Background and education

Wood received dual undergraduate degrees in Biology and Chemical Engineering from the California Institute of Technology in 1990. He worked as an undergraduate in the lab led by Dr. Frances H. Arnold. He earned his Ph.D. in chemical engineering from Rensselaer Polytechnic Institute in 2000. His doctoral co-advisors were Dr. Georges Belfort at Rensselaer Polytechnic Institute, Troy, NY, and Dr. Marlene Belfort at Wadsworth Center, New York State Department of Health, Albany, NY.

Career

After finishing undergraduate dual degrees in biology and chemical engineering at Caltech in 1990, Wood started working on high-viscosity mucopolysaccharide fermentation development at CP Kelco located on the San Diego Bay in San Diego, California. Soon after, he joined Amgen in 1991, the same year that Amgen received FDA approval for Neupogen®. This recombinant protein cytokine drug induces white blood cell production to fight infections in immunocompromised cancer patients after undergoing chemotherapy or radiation treatment.[3] Wood worked on the GMP fermentation team to manufacture Neupogen® (rhG-CSF) between 1991-1993. As it is known today, Neupogen® became one of the most successful biotech drugs at that time and second blockbuster for Amgen after Epogen®. In 1993, Wood joined the group of Dr. Georges Belfort[4] as a Ph.D. student at Rensselaer Polytechnic Institute, where he worked primarily on mini-intein development for protein purification. During this time, he engineered the ∆I-CM intein, which was derived from Mycobacterium tuberculosis recA intein.[1][5][6][7] After earning his Ph.D. he joined as a postdoctorate a team at Bristol Myers Squibb in Hopewell, New Jersey. This time he focused on the recovery and in vitro processing of transgenic monoclonal antibody therapeutics. In 2001, Dr. Wood started his academic career at Princeton University as an Assistant Professor of Chemical and Biological Engineering, where he continued his research focusing on self-removing tags, protein engineering, and applied biosensors.[8][9][10][11][12] In 2009, he joined Chemical and Biomolecular Engineering Department at Ohio State University as an Associate Professor and soon after was appointed as a Full Professor. While at the Ohio State University he continued expanding his research on self-removing tags to explore additional applications, including the development of multitarget sRNAs that can be used for metabolic engineering, and the modification of human butyrylcholinesterase for the degradation of the chemical warfare nerve agents in collaboration with the Battelle Memorial Institute.[13][14] Currently, he has joint appointments with Department of Chemistry and Biochemistry and the Molecular Biophysics Training Program at the Ohio State University. He is one of a small group of researches worldwide focusing on intein implementation in various applications, along with Dr. Marlene Belfort and Dr. Tom Muir.[15][16]


Dr. Wood's research focuses on developing new technologies by recombining protein domains, particularly in biopharmaceutical development and manufacturing. Over the past several years, he has continued refining these methods for biopharmaceutical development and manufacturing, and his work has drawn funding from the DARPA BioMOD project (in collaboration with Dr. Govind Rao and Dr. Doug Frey Labs at UMBC), NIH, NSF and US Army Research Office, projects as well as several industry sponsors.[17][18][19][20][21][22][23] Dr. Wood was also involved in the development of protein switches for biotechnology funded by the NSF Career Award.[24]

Dr. Wood is an author of over 60 publications, six issued patents, two additional patent applications pending and an additional dozen book chapters or edited volumes. His publications have been cited nearly 4000 times.[25]


Since Dr. Wood's research intersects with chemical engineering, molecular biology, biopharmaceutical development and manufacturing, his former undergraduate and graduate students have mostly entered industry positions at the top companies, which include Amgen, Genentech, Novartis, Biogen, Regeneron, Merck, WuXi Biologics, Shire Pharmaceuticals, Proctor & Gamble, Genzyme, GlaxoSmithKline, EirGenix, and Pfizer. Other former students have become professors at universities that include UT Austin, UC Irvine, Bucknell University, Smith College, The George Washington University, North Carolina State University, and the NYU School of Medicine. During the interview about careers in bioprocessing field for Science Magazine, he said "My primary advice to anyone considering this field is to take as many courses in engineering and biology as possible".[26]

Wood is a member of the American Chemical Society, BIOT division, American Institute of Chemical Engineers, and International Society of Pharmaceutical Engineers.

Awards and honors

  • 2003: NSF Career Award[24]
  • 2016: Lumley Engineering Research Award, Ohio State College of Engineering[27]

Founder of Protein Capture Science

Protein Capture Science[28] is a biotech start-up company commercializing a self-removing affinity tag technology for research and large-scale purifications of proteins.[29][30] Dr. Wood and Dr. Izabela Gierach are co-founders of the company, based in Columbus, Ohio. The first line of the iCapTag™ products for protein purification was showcased in the Industry Innovators 2021-2022 Issue of the journal BioProcess International.[31] Protein Capture Science was recently awarded several grants and funding, including highly competitive The Ohio Third Frontier Technology Validation and Start-Up Fund and the Concept Fund for extraordinary technologies by the Department of Development, State of Ohio.[32]

External Links

References

  1. ^ a b Wood, David W.; Wu, Wei; Belfort, Georges; Derbyshire, Victoria; Belfort, Marlene (1999). "A genetic system yields self-cleaving inteins for bioseparations". Nature Biotechnology. 17 (9): 889–892. doi:10.1038/12879. ISSN 1546-1696.
  2. ^ 6933362, Belfort, Marlene; Belfort, Georges & Derbyshire, Vicky et al., "United States Patent: 6933362 - GENETIC SYSTEM AND SELF-CLEAVING INTEINS DERIVED THEREFROM, BIOSEPARATIONS AND PROTEIN PURIFICATION EMPLOYING SAME, AND METHODS FOR DETERMINING CRITICAL, GENERALIZABLE AMINO ACID RESIDUES FOR VARYING INTEIN ACTIVITY", issued August 23, 2005 
  3. ^ "Amgen History". Amgen, Inc. Retrieved 2022-07-27.
  4. ^ "Georges Belfort | The Howard P. Isermann Department of Chemical and Biological Engineering". cbe.rpi.edu. Retrieved 2022-07-27.
  5. ^ Derbyshire, Victoria; Wood, David W.; Wu, Wei; Dansereau, John T.; Dalgaard, Jacob Z.; Belfort, Marlene (1997-10-14). "Genetic definition of a protein-splicing domain: Functional mini-inteins support structure predictions and a model for intein evolution". Proceedings of the National Academy of Sciences. 94 (21): 11466–11471. doi:10.1073/pnas.94.21.11466. ISSN 0027-8424. PMC 23508. PMID 9326633.{{cite journal}}: CS1 maint: PMC format (link)
  6. ^ Wood, D.W.; Derbyshire, V.; Wu, W.; Chartrain, M.; Belfort, M.; Belfort, G. (2000-12-01). "Optimized Single-Step Affinity Purification with a Self-Cleaving Intein Applied to Human Acidic Fibroblast Growth Factor". Biotechnology Progress. 16 (6): 1055–1063. doi:10.1021/bp0000858. ISSN 8756-7938.
  7. ^ "Generation and application of a self -cleaving protein linker for use in single-step affinity fusion based protein purification - ProQuest". www.proquest.com. Retrieved 2022-07-27.
  8. ^ Wu, Wan-Yi; Mee, Courtney; Califano, Filomena; Banki, Reza; Wood, David W. (2006). "Recombinant protein purification by self-cleaving aggregation tag". Nature Protocols. 1 (5): 2257–2262. doi:10.1038/nprot.2006.314. ISSN 1750-2799.
  9. ^ Skretas, G. (2005-02-01). "Regulation of protein activity with small-molecule-controlled inteins". Protein Science. 14 (2): 523–532. doi:10.1110/ps.04996905. ISSN 0961-8368. PMC 2386410. PMID 15632292.{{cite journal}}: CS1 maint: PMC format (link)
  10. ^ Skretas, Georgios; Meligova, Aggeliki K.; Villalonga-Barber, Carolina; Mitsiou, Dimitra J.; Alexis, Michael N.; Micha-Screttas, Maria; Steele, Barry R.; Screttas, Constantinos G.; Wood, David W. (2007-07-01). "Engineered Chimeric Enzymes as Tools for Drug Discovery: Generating Reliable Bacterial Screens for the Detection, Discovery, and Assessment of Estrogen Receptor Modulators". Journal of the American Chemical Society. 129 (27): 8443–8457. doi:10.1021/ja067754j. ISSN 0002-7863.
  11. ^ Hartman, Izabela; Gillies, Alison R.; Arora, Sonia; Andaya, Christina; Royapet, Nitya; Welsh, William J.; Wood, David W.; Zauhar, Randy J. (2009-10-01). "Application of Screening Methods, Shape Signatures and Engineered Biosensors in Early Drug Discovery Process". Pharmaceutical Research. 26 (10): 2247–2258. doi:10.1007/s11095-009-9941-z. ISSN 1573-904X.
  12. ^ Li, Jingjing; Gierach, Izabela; Gillies, Alison R.; Warden, Charles D.; Wood, David W. (2011-11-15). "Engineering and optimization of an allosteric biosensor protein for peroxisome proliferator-activated receptor γ ligands". Biosensors and Bioelectronics. 29 (1): 132–139. doi:10.1016/j.bios.2011.08.006. ISSN 0956-5663.
  13. ^ Lahiry, Ashwin; Stimple, Samuel D.; Wood, David W.; Lease, Richard A. (2017-04-21). "Retargeting a Dual-Acting sRNA for Multiple mRNA Transcript Regulation". ACS Synthetic Biology. 6 (4): 648–658. doi:10.1021/acssynbio.6b00261. ISSN 2161-5063.
  14. ^ McGarry, Kevin G; Lalisse, Remy F; Moyer, Robert A; Johnson, Kristyn M; Tallan, Alexi M; Winters, Tyson P; Taris, Joeseph E; McElroy, Craig A; Lemmon, Erin E; Shafaat, Hannah S; Fan, Yamin (2020-03-01). "A Novel, Modified Human Butyrylcholinesterase Catalytically Degrades the Chemical Warfare Nerve Agent, Sarin". Toxicological Sciences. 174 (1): 133–146. doi:10.1093/toxsci/kfz251. ISSN 1096-6080.
  15. ^ Prabhala, Sai Vivek; Gierach, Izabela; Wood, David W. (2022). "The Evolution of Intein-Based Affinity Methods as Reflected in 30 years of Patent History". Frontiers in Molecular Biosciences. 9. doi:10.3389/fmolb.2022.857566/full. ISSN 2296-889X.{{cite journal}}: CS1 maint: unflagged free DOI (link)
  16. ^ Wood, David W.; Harcum, Sarah W.; Belfort, Georges (2005), Belfort, Marlene; Wood, David W.; Stoddard, Barry L.; Derbyshire, Victoria (eds.), "Industrial Applications of Intein Technology", Homing Endonucleases and Inteins, Berlin, Heidelberg: Springer, pp. 345–364, doi:10.1007/3-540-29474-0_20, ISBN 978-3-540-29474-0, retrieved 2022-07-27
  17. ^ "NSF Award Search: Award # 1264322 - Highly Controllable Self-cleaving Tags for Biopharmaceutical Research and Manufacturing Purification Platforms". www.nsf.gov. Retrieved 2022-07-27.
  18. ^ "NSF Award Search: Award # 1264322 - Highly Controllable Self-cleaving Tags for Biopharmaceutical Research and Manufacturing Purification Platforms". www.nsf.gov. Retrieved 2022-07-27.
  19. ^ "Just-in-time therapeutics manufacturing project receives additional DARPA funding". COLLEGE OF ENGINEERING. 2015-07-28. Retrieved 2022-07-27.
  20. ^ "Graduate students in the Frey Laboratory at UMBC". userpages.umbc.edu. Retrieved 2022-07-27.
  21. ^ "Ohio State engineering faculty attract $3.3 million in NSF funding in June". COLLEGE OF ENGINEERING. 2013-07-03. Retrieved 2022-07-27.
  22. ^ "Advancing the science and engineering of artificial blood". Chemical and Biomolecular Engineering. 2021-02-22. Retrieved 2022-07-27.
  23. ^ Banki, Mahmoud Reza; Feng, Liang; Wood, David W. (2005). "Simple bioseparations using self-cleaving elastin-like polypeptide tags". Nature Methods. 2 (9): 659–662. doi:10.1038/nmeth787. ISSN 1548-7105.
  24. ^ a b "NSF Award Search: Award # 0348220 - CAREER: Protein Switches for Molecular Biotechnology". www.nsf.gov. Retrieved 2022-07-27.
  25. ^ "David W. Wood". scholar.google.com. Retrieved 2022-07-27.
  26. ^ "Melding talents with a career in bioprocessing". www.science.org. Retrieved 2022-07-27.
  27. ^ "David Wood's research recognized with Lumley Award". Chemical and Biomolecular Engineering. 2016-04-18. Retrieved 2022-07-27.
  28. ^ "Protein Capture Science". www.proteincapturescience.com. Retrieved 2022-07-27.
  29. ^ "Protein Capture Science Seeks to Accelerate Biomedical Research". Rev1 Ventures. 2021-05-20. Retrieved 2022-07-27.
  30. ^ "Protein Capture Science Accelerates Medical Therapies". Rev1 Ventures. 2022-04-15. Retrieved 2022-07-27.
  31. ^ "iCapTag Tagless Protein Purification Platform". BioProcess International. 2021-08-25. Retrieved 2022-07-27.
  32. ^ Ohio Advancing Cutting-Edge Technology, Third Frontier Accelerating New Ideas and Solutions.https://development.ohio.gov/static/business/thirdfrontier/2020%2006%2024%20Ohio%20Advancing%20Cutting-Edge%20Technology.pdf. Retrieved 2022-07-27
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