NSP1 (rotavirus): Difference between revisions
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NSP1, the product of rotavirus gene 5, is an nonstructural RNA-binding protein that contains a cysteine-rich region and is a component of early replication intermediates. RNA-folding predictions suggest that this region of the NSP1 mRNA can interact with itself, producing a stem-loop structure similar to that found near the 5'-terminus of the NSP1 mRNA.[1]
The carboxyl-half of the rotavirus nonstructural protein NSP1 is not required for virus replication.[2]
NSP1 could play a role in host range restriction.[3]
The cysteine-rich region of NSP1 is not considered essential for genome segment reassortment with heterologous virus.[4]
NSP1, interacts with IRF3 in the infected cell. NSP1 is an antagonist of the IFN-signaling pathway.[5]
Interferon regulatory factor 3 (IRF3) is a key transcription factor involved in the induction of interferon (IFN) in response to viral infection. NSP1 binds to and targets IRF3 for proteasome degradation early post-infection. IRF3 degradation is dependent on the presence of NSP1 and the integrity of the N-terminal zinc-binding domain, coupled with the regulated stability of IRF3 and NSP1 by the proteasome, collectively support the hypothesis that NSP1 is an E3 ubiquitin ligase.[6]
NSP1 could mediates the degradation of IRF3, IRF5, and IRF7 by recognizing a common element of IRF proteins, thereby allowing NSP1 to act as a broad-spectrum antagonist of IRF function.[7]
- ^ Hua, J (1993- Sep). "Comparative analysis of the rotavirus NS53 gene: conservation of basic and cysteine-rich regions in the protein and possible stem-loop structures in the RNA". Virology. 196 (1): 372–8. 8395125 doi:10.1006/viro.1993.1492. Retrieved 2007-11-02.
{{cite journal}}: Check date values in:|date=(help); Unknown parameter|coauthors=ignored (|author=suggested) (help) - ^ Hua, J (1994- Feb). "The carboxyl-half of the rotavirus nonstructural protein NS53 (NSP1) is not required for virus replication". Virology. 198 (2): 567–76. 8291239 doi:10.1006/viro.1994.1068. Retrieved 2007-11-02.
{{cite journal}}: Check date values in:|date=(help); Unknown parameter|coauthors=ignored (|author=suggested) (help) - ^ Dunn, S J (1994- Aug- 15). "Comparison of the rotavirus nonstructural protein NSP1 (NS53) from different species by sequence analysis and northern blot hybridization". Virology. 203 (1): 178–83. 8030275 doi:10.1006/viro.1994.1471. Retrieved 2007-11-02.
{{cite journal}}: Check date values in:|date=(help); Unknown parameter|coauthors=ignored (|author=suggested) (help) - ^ Okada, J (1999). "Analysis on reassortment of rotavirus NSP1 genes lacking coding region for cysteine-rich zinc finger motif". Archives of virology. 144 (2): 345–53. 10470258. Retrieved 2007-11-02.
{{cite journal}}: Unknown parameter|coauthors=ignored (|author=suggested) (help) - ^ Barro, Mario (2005- Mar- 15). "Rotavirus nonstructural protein 1 subverts innate immune response by inducing degradation of IFN regulatory factor 3". Proceedings of the National Academy of Sciences of the United States of America. 102 (11): 4114–9. 15741273 doi:10.1073/pnas.0408376102. Retrieved 2007-11-02.
{{cite journal}}: Check date values in:|date=(help); Unknown parameter|coauthors=ignored (|author=suggested) (help) - ^ Graff, Joel W (2007- Feb). "Zinc-binding domain of rotavirus NSP1 is required for proteasome-dependent degradation of IRF3 and autoregulatory NSP1 stability". The Journal of general virology. 88 (Pt 2): 613–20. 17251580 doi:10.1099/vir.0.82255-0. Retrieved 2007-11-02.
{{cite journal}}: Check date values in:|date=(help); Unknown parameter|coauthors=ignored (|author=suggested) (help) - ^ Barro, Mario (2007- May). "Rotavirus NSP1 inhibits expression of type I interferon by antagonizing the function of interferon regulatory factors IRF3, IRF5, and IRF7". Journal of virology. 81 (9): 4473–81. 17301153 doi:10.1128/JVI.02498-06. Retrieved 2007-11-02.
{{cite journal}}: Check date values in:|date=(help); Unknown parameter|coauthors=ignored (|author=suggested) (help)
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