Ki-67 (protein): Difference between revisions
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{{PBB|geneid=4288}} |
{{PBB|geneid=4288}} |
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'''Antigen identified by monoclonal antibody Ki-67''' also known as '''Ki-67''' or '''MKI67''' is a [[protein]] which in humans is encoded by the ''MKI67'' gene.<ref name="entrez">{{cite web | title = Entrez Gene: Antigen identified by monoclonal antibody Ki-67 | url =http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=4288 | accessdate = }}</ref> |
'''Antigen identified by monoclonal antibody Ki-67''' also known as '''Ki-67''' or '''MKI67''' is a [[protein]] which in humans is encoded by the ''MKI67'' gene.<ref name="entrez">{{cite web | title = Entrez Gene: Antigen identified by monoclonal antibody Ki-67 | url =http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=4288 | accessdate = }}</ref><ref name="pmid2571566">{{cite journal | author = Schonk DM, Kuijpers HJ, van Drunen E, van Dalen CH, Geurts van Kessel AH, Verheijen R, Ramaekers FC | title = Assignment of the gene(s) involved in the expression of the proliferation-related Ki-67 antigen to human chromosome 10 | journal = Hum. Genet. | volume = 83 | issue = 3 | pages = 297–9 | year = 1989 | month = October | pmid = 2571566 | doi = | url = | issn = }}</ref><ref name="pmid16206250">{{cite journal | author = Bullwinkel J, Baron-Lühr B, Lüdemann A, Wohlenberg C, Gerdes J, Scholzen T | title = Ki-67 protein is associated with ribosomal RNA transcription in quiescent and proliferating cells | journal = J. Cell. Physiol. | volume = 206 | issue = 3 | pages = 624–35 | year = 2006 | month = March | pmid = 16206250 | doi = 10.1002/jcp.20494 | url = | issn = }}</ref> |
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==Use as cell marker== |
==Use as cell marker== |
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The '''Ki-67''' protein (also known as or '''MKI67''') is a cellular marker for proliferation. It is strictly associated with [[cell proliferation]]. During [[interphase]], the Ki-67 [[antigen]] can be exclusively detected within the [[cell nucleus]], whereas in [[mitosis]] most of the protein is relocated to the surface of the |
The '''Ki-67''' protein (also known as or '''MKI67''') is a cellular marker for proliferation.<ref name="pmid10653597">{{cite journal | author = Scholzen T, Gerdes J | title = The Ki-67 protein: from the known and the unknown | journal = J. Cell. Physiol. | volume = 182 | issue = 3 | pages = 311–22 | year = 2000 | month = March | pmid = 10653597 | doi = 10.1002/(SICI)1097-4652(200003)182:3<311::AID-JCP1>3.0.CO;2-9 | url = | issn = }}</ref> It is strictly associated with [[cell proliferation]]. During [[interphase]], the Ki-67 [[antigen]] can be exclusively detected within the [[cell nucleus]], whereas in [[mitosis]] most of the protein is relocated to the surface of the [[chromosome]]s. Ki-67 protein is present during all active phases of the [[cell cycle]] (G<sub>1</sub>, S, G<sub>2</sub>, and mitosis), but is absent from resting cells (G<sub>0</sub>). Ki-67 is an excellent marker to determine the growth fraction of a given cell population. The fraction of Ki-67-positive [[tumor]] cells (the ''Ki-67 labelling index'') is often correlated with the clinical course of [[cancer]]. The best-studied examples in this context are [[carcinoma]]s of the [[prostate]] and the [[breast]]. For these types of tumors, the prognostic value for survival and tumor recurrence have repeatedly been proven in uni- and multivariate analysis. |
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[[chromosome]]s. Ki-67 protein is present during all active phases of the [[cell cycle]] (G<sub>1</sub>, S, G<sub>2</sub>, and mitosis), but is absent from resting cells (G<sub>0</sub>). Ki-67 is an excellent marker to determine the growth fraction of a given cell population. The fraction of Ki-67-positive [[tumor]] cells (the ''Ki-67 labelling index'') is often correlated with the clinical course of [[cancer]]. The best-studied examples in this context are [[carcinoma]]s of the [[prostate]] and the [[breast]]. For these types of tumors, the prognostic value for survival and tumor recurrence have repeatedly been proven in uni- and multivariate analysis. |
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The Ki-67 protein was originally defined by the prototype monoclonal [[antibody]] Ki-67,<ref name = "pmid6339421">{{cite journal |author=Gerdes J, Schwab U, Lemke H, Stein H |title=Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation |journal=Int. J. Cancer |volume=31 |issue=1 |pages=13–20 |year=1983 |pmid=6339421 |url=http://www3.interscience.wiley.com/cgi-bin/abstract/112713361/ |doi=10.1002/ijc.2910310104}}</ref> which was generated by immunizing mice with nuclei of the [[Hodgkin lymphoma]] cell line L428. The name is derived from the city of origin ([[Kiel]]) and the number of the original clone in the 96-well plate. |
The Ki-67 protein was originally defined by the prototype monoclonal [[antibody]] Ki-67,<ref name = "pmid6339421">{{cite journal |author=Gerdes J, Schwab U, Lemke H, Stein H |title=Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation |journal=Int. J. Cancer |volume=31 |issue=1 |pages=13–20 |year=1983 |pmid=6339421 |url=http://www3.interscience.wiley.com/cgi-bin/abstract/112713361/ |doi=10.1002/ijc.2910310104}}</ref> which was generated by immunizing mice with nuclei of the [[Hodgkin lymphoma]] cell line L428. The name is derived from the city of origin ([[Kiel]]) and the number of the original clone in the 96-well plate. |
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==References== |
==References== |
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{{reflist}} |
{{reflist}} |
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==Literature== |
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*{{cite journal |author=Scholzen T, Gerdes J |title=The Ki-67 protein: from the known and the unknown |journal=J. Cell. Physiol. |volume=182 |issue=3 |pages=311–22 |year=2000 |pmid=10653597 |url=http://www3.interscience.wiley.com/cgi-bin/abstract/69501961/ |doi=10.1002/(SICI)1097-4652(200003)182:3<311::AID-JCP1>3.0.CO;2-9 |doilabel=10.1002/(SICI)1097-4652(200003)182:3<311::AID-JCP1>3.0.CO;2-9}} (Review) |
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==External links== |
==External links== |
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Revision as of 18:20, 15 November 2008
Template:PBB Antigen identified by monoclonal antibody Ki-67 also known as Ki-67 or MKI67 is a protein which in humans is encoded by the MKI67 gene.[1][2][3]
Use as cell marker
The Ki-67 protein (also known as or MKI67) is a cellular marker for proliferation.[4] It is strictly associated with cell proliferation. During interphase, the Ki-67 antigen can be exclusively detected within the cell nucleus, whereas in mitosis most of the protein is relocated to the surface of the chromosomes. Ki-67 protein is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells (G0). Ki-67 is an excellent marker to determine the growth fraction of a given cell population. The fraction of Ki-67-positive tumor cells (the Ki-67 labelling index) is often correlated with the clinical course of cancer. The best-studied examples in this context are carcinomas of the prostate and the breast. For these types of tumors, the prognostic value for survival and tumor recurrence have repeatedly been proven in uni- and multivariate analysis.
The Ki-67 protein was originally defined by the prototype monoclonal antibody Ki-67,[5] which was generated by immunizing mice with nuclei of the Hodgkin lymphoma cell line L428. The name is derived from the city of origin (Kiel) and the number of the original clone in the 96-well plate.
MIB-1 is a commonly used monoclonal antibody that detects the Ki-67 antigen. It is used in clinical applications to determine the Ki-67 labelling index. One of its primary advantages over the original Ki-67 antibody (and the reason why it has essentially supplanted the original antibody for clinical use) is that it can be used on formalin-fixed paraffin-embedded sections, after heat-mediated antigen retrieval.
See also
- PCNA - Proliferating Cell Nuclear Antigen, expressed during the DNA synthesis.
References
- ^ "Entrez Gene: Antigen identified by monoclonal antibody Ki-67".
- ^ Schonk DM, Kuijpers HJ, van Drunen E, van Dalen CH, Geurts van Kessel AH, Verheijen R, Ramaekers FC (1989). "Assignment of the gene(s) involved in the expression of the proliferation-related Ki-67 antigen to human chromosome 10". Hum. Genet. 83 (3): 297–9. PMID 2571566.
{{cite journal}}: Unknown parameter|month=ignored (help)CS1 maint: multiple names: authors list (link) - ^ Bullwinkel J, Baron-Lühr B, Lüdemann A, Wohlenberg C, Gerdes J, Scholzen T (2006). "Ki-67 protein is associated with ribosomal RNA transcription in quiescent and proliferating cells". J. Cell. Physiol. 206 (3): 624–35. doi:10.1002/jcp.20494. PMID 16206250.
{{cite journal}}: Unknown parameter|month=ignored (help)CS1 maint: multiple names: authors list (link) - ^ Scholzen T, Gerdes J (2000). "The Ki-67 protein: from the known and the unknown". J. Cell. Physiol. 182 (3): 311–22. doi:10.1002/(SICI)1097-4652(200003)182:3<311::AID-JCP1>3.0.CO;2-9. PMID 10653597.
{{cite journal}}: Unknown parameter|month=ignored (help) - ^ Gerdes J, Schwab U, Lemke H, Stein H (1983). "Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation". Int. J. Cancer. 31 (1): 13–20. doi:10.1002/ijc.2910310104. PMID 6339421.
{{cite journal}}: CS1 maint: multiple names: authors list (link)
External links
- Ki-67+Antigen at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
 | This article on a gene on human chromosome 10 is a stub. You can help Wikipedia by expanding it. |