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'''Φ29 DNA polymerase''' is an enzyme from the [[Φ29 phage|bacteriophage Φ29]]. It is being increasingly used in [[molecular biology]] for [[Multiple displacement amplification|multiple displacement DNA amplification]] procedures, and has a number of features that make it particularly suitable for this application.
'''Φ29 DNA polymerase''' is an enzyme from the [[Φ29 phage|bacteriophage Φ29]]. It is being increasingly used in [[molecular biology]] for [[Multiple displacement amplification|multiple displacement DNA amplification]] procedures, and has a number of features that make it particularly suitable for this application.

==Further reading==
<div style='display:none'>
<ref name="pmid20926164">{{cite journal |author=Linck L, Resch-Genger U |title=Identification of efficient fluorophores for the direct labeling of DNA via rolling circle amplification (RCA) polymerase φ29. |journal=Eur J Med Chem |volume= |issue= |pages= |year=2010 |pmid=20926164}}</ref>
<ref name="pmid20823261">{{cite journal |author=de Vega M, Lázaro JM, Mencía M, Blanco L, Salas M |title=Improvement of φ29 DNA polymerase amplification performance by fusion of DNA binding motifs. |journal=Proc Natl Acad Sci U S A |volume=107 |issue=38 |pages=16506-11 |year=2010 |pmid=20823261}}</ref>
<ref name="pmid19883660">{{cite journal |author=Pérez-Arnaiz P, Lázaro JM, Salas M, de Vega M |title=phi29 DNA polymerase active site: role of residue Val250 as metal-dNTP complex ligand and in protein-primed initiation. |journal=J Mol Biol |volume=395 |issue=2 |pages=223-33 |year=2010 |pmid=19883660}}</ref>
<ref name="pmid19576228">{{cite journal |author=Pérez-Arnaiz P, Lázaro JM, Salas M, de Vega M |title=Functional importance of bacteriophage phi29 DNA polymerase residue Tyr148 in primer-terminus stabilisation at the 3'-5' exonuclease active site. |journal=J Mol Biol |volume=391 |issue=5 |pages=797-807 |year=2009 |pmid=19576228}}</ref>
<ref name="pmid19375325">{{cite journal |author=Johne R, Müller H, Rector A, van Ranst M, Stevens H |title=Rolling-circle amplification of viral DNA genomes using phi29 polymerase. |journal=Trends Microbiol |volume=17 |issue=5 |pages=205-11 |year=2009 |pmid=19375325}}</ref>
<ref name="pmid19309528">{{cite journal |author=Alsmadi O, Alkayal F, Monies D, Meyer BF |title=Specific and complete human genome amplification with improved yield achieved by phi29 DNA polymerase and a novel primer at elevated temperature. |journal=BMC Res Notes |volume=2 |issue= |pages=48 |year=2009 |pmid=19309528}}</ref>
<ref name="pmid19244362">{{cite journal |author=Lagunavicius A, Merkiene E, Kiveryte Z, Savaneviciute A, Zimbaite-Ruskuliene V, Radzvilavicius T, Janulaitis A |title=Novel application of Phi29 DNA polymerase: RNA detection and analysis in vitro and in situ by target RNA-primed RCA. |journal=RNA |volume=15 |issue=5 |pages=765-71 |year=2009 |pmid=19244362}}</ref>
<ref name="pmid19033368">{{cite journal |author=Rodríguez I, Lázaro JM, Salas M, de Vega M |title=Involvement of the TPR2 subdomain movement in the activities of phi29 DNA polymerase. |journal=Nucleic Acids Res |volume=37 |issue=1 |pages=193-203 |year=2009 |pmid=19033368}}</ref>
<ref name="pmid18939810">{{cite journal |author=Sahu S, LaBean TH, Reif JH |title=A DNA nanotransport device powered by polymerase phi29. |journal=Nano Lett |volume=8 |issue=11 |pages=3870-8 |year=2008 |pmid=18939810}}</ref>
<ref name="pmid18755142">{{cite journal |author=Xu Y, Gao S, Bruno JF, Luft BJ, Dunn JJ |title=Rapid detection and identification of a pathogen's DNA using Phi29 DNA polymerase. |journal=Biochem Biophys Res Commun |volume=375 |issue=4 |pages=522-5 |year=2008 |pmid=18755142}}</ref>
<ref name="pmid18533898">{{cite journal |author=Kumar G, Garnova E, Reagin M, Vidali A |title=Improved multiple displacement amplification with phi29 DNA polymerase for genotyping of single human cells. |journal=Biotechniques |volume=44 |issue=7 |pages=879-90 |year=2008 |pmid=18533898}}</ref>
<ref name="pmid18379997">{{cite journal |author=Salas M, Blanco L, Lázaro JM, de Vega M |title=The bacteriophage phi29 DNA polymerase. |journal=IUBMB Life |volume=60 |issue=1 |pages=82-5 |year=2008 |pmid=18379997}}</ref>
<ref name="pmid18370092">{{cite journal |author=Silander K, Saarela J |title=Whole genome amplification with Phi29 DNA polymerase to enable genetic or genomic analysis of samples of low DNA yield. |journal=Methods Mol Biol |volume=439 |issue= |pages=1-18 |year=2008 |pmid=18370092}}</ref>
<ref name="pmid18230765">{{cite journal |author=Lagunavicius A, Kiveryte Z, Zimbaite-Ruskuliene V, Radzvilavicius T, Janulaitis A |title=Duality of polynucleotide substrates for Phi29 DNA polymerase: 3'-->5' RNase activity of the enzyme. |journal=RNA |volume=14 |issue=3 |pages=503-13 |year=2008 |pmid=18230765}}</ref>
<ref name="pmid17913744">{{cite journal |author=Pérez-Arnaiz P, Longás E, Villar L, Lázaro JM, Salas M, de Vega M |title=Involvement of phage phi29 DNA polymerase and terminal protein subdomains in conferring specificity during initiation of protein-primed DNA replication. |journal=Nucleic Acids Res |volume=35 |issue=21 |pages=7061-73 |year=2007 |pmid=17913744}}</ref>
<ref name="pmid17611604">{{cite journal |author=Berman AJ, Kamtekar S, Goodman JL, Lázaro JM, de Vega M, Blanco L, Salas M, Steitz TA |title=Structures of phi29 DNA polymerase complexed with substrate: the mechanism of translocation in B-family polymerases. |journal=EMBO J |volume=26 |issue=14 |pages=3494-505 |year=2007 |pmid=17611604}}</ref>
<ref name="pmid17226067">{{cite journal |author=Knierim D, Maiss E |title=Application of Phi29 DNA polymerase in identification and full-length clone inoculation of tomato yellow leaf curl Thailand virus and tobacco leaf curl Thailand virus. |journal=Arch Virol |volume=152 |issue=5 |pages=941-54 |year=2007 |pmid=17226067}}</ref>
<ref name="pmid17174409">{{cite journal |author=Owor BE, Shepherd DN, Taylor NJ, Edema R, Monjane AL, Thomson JA, Martin DP, Varsani A |title=Successful application of FTA Classic Card technology and use of bacteriophage phi29 DNA polymerase for large-scale field sampling and cloning of complete maize streak virus genomes. |journal=J Virol Methods |volume=140 |issue=1-2 |pages=100-5 |year=2007 |pmid=17174409}}</ref>
<ref name="pmid17150521">{{cite journal |author=Sato M, Ohtsuka M, Ohmi Y |title=Repeated GenomiPhi, phi29 DNA polymerase-based rolling circle amplification, is useful for generation of large amounts of plasmid DNA. |journal=Nucleic Acids Symp Ser (Oxf) |volume= |issue=48 |pages=147-8 |year=2004 |pmid=17150521}}</ref>
<ref name="pmid16757576">{{cite journal |author=Pérez-Arnaiz P, Lázaro JM, Salas M, de Vega M |title=Involvement of phi29 DNA polymerase thumb subdomain in the proper coordination of synthesis and degradation during DNA replication. |journal=Nucleic Acids Res |volume=34 |issue=10 |pages=3107-15 |year=2006 |pmid=16757576}}</ref>
<ref name="pmid16511564">{{cite journal |author=Kamtekar S, Berman AJ, Wang J, Lázaro JM, de Vega M, Blanco L, Salas M, Steitz TA |title=The phi29 DNA polymerase:protein-primer structure suggests a model for the initiation to elongation transition. |journal=EMBO J |volume=25 |issue=6 |pages=1335-43 |year=2006 |pmid=16511564}}</ref>
<ref name="pmid16286637">{{cite journal |author=Hutchison CA, Smith HO, Pfannkoch C, Venter JC |title=Cell-free cloning using phi29 DNA polymerase. |journal=Proc Natl Acad Sci U S A |volume=102 |issue=48 |pages=17332-6 |year=2005 |pmid=16286637}}</ref>
<ref name="pmid16023891">{{cite journal |author=Sato M, Ohtsuka M, Ohmi Y |title=Usefulness of repeated GenomiPhi, a phi29 DNA polymerase-based rolling circle amplification kit, for generation of large amounts of plasmid DNA. |journal=Biomol Eng |volume=22 |issue=4 |pages=129-32 |year=2005 |pmid=16023891}}</ref>
<ref name="pmid15845765">{{cite journal |author=Rodríguez I, Lázaro JM, Blanco L, Kamtekar S, Berman AJ, Wang J, Steitz TA, Salas M, de Vega M |title=A specific subdomain in phi29 DNA polymerase confers both processivity and strand-displacement capacity. |journal=Proc Natl Acad Sci U S A |volume=102 |issue=18 |pages=6407-12 |year=2005 |pmid=15845765}}</ref>
<ref name="pmid15777661">{{cite journal |author=Truniger V, Bonnin A, Lázaro JM, de Vega M, Salas M |title=Involvement of the "linker" region between the exonuclease and polymerization domains of phi29 DNA polymerase in DNA and TP binding. |journal=Gene |volume=348 |issue= |pages=89-99 |year=2005 |pmid=15777661}}</ref>
<ref name="pmid15721296">{{cite journal |author=Umetani N, de Maat MF, Mori T, Takeuchi H, Hoon DS |title=Synthesis of universal unmethylated control DNA by nested whole genome amplification with phi29 DNA polymerase. |journal=Biochem Biophys Res Commun |volume=329 |issue=1 |pages=219-23 |year=2005 |pmid=15721296}}</ref>
<ref name="pmid15621421">{{cite journal |author=Gadkar V, Rillig MC |title=Application of Phi29 DNA polymerase mediated whole genome amplification on single spores of arbuscular mycorrhizal (AM) fungi. |journal=FEMS Microbiol Lett |volume=242 |issue=1 |pages=65-71 |year=2005 |pmid=15621421}}</ref>
<ref name="pmid15546620">{{cite journal |author=Kamtekar S, Berman AJ, Wang J, Lázaro JM, de Vega M, Blanco L, Salas M, Steitz TA |title=Insights into strand displacement and processivity from the crystal structure of the protein-primed DNA polymerase of bacteriophage phi29. |journal=Mol Cell |volume=16 |issue=4 |pages=609-18 |year=2004 |pmid=15546620}}</ref>
<ref name="pmid15168072">{{cite journal |author=Adachi E, Shimamura K, Wakamatsu S, Kodama H |title=Amplification of plant genomic DNA by Phi29 DNA polymerase for use in physical mapping of the hypermethylated genomic region. |journal=Plant Cell Rep |volume=23 |issue=3 |pages=144-7 |year=2004 |pmid=15168072}}</ref>
<ref name="pmid15033354">{{cite journal |author=Rodríguez I, Lázaro JM, Salas M, De Vega M |title=phi29 DNA polymerase-terminal protein interaction. Involvement of residues specifically conserved among protein-primed DNA polymerases. |journal=J Mol Biol |volume=337 |issue=4 |pages=829-41 |year=2004 |pmid=15033354}}</ref>
<ref name="pmid14738990">{{cite journal |author=Inoue-Nagata AK, Albuquerque LC, Rocha WB, Nagata T |title=A simple method for cloning the complete begomovirus genome using the bacteriophage phi29 DNA polymerase. |journal=J Virol Methods |volume=116 |issue=2 |pages=209-11 |year=2004 |pmid=14738990}}</ref>
<ref name="pmid14729920">{{cite journal |author=Truniger V, Lázaro JM, Salas M |title=Function of the C-terminus of phi29 DNA polymerase in DNA and terminal protein binding. |journal=Nucleic Acids Res |volume=32 |issue=1 |pages=361-70 |year=2004 |pmid=14729920}}</ref>
<ref name="pmid14672657">{{cite journal |author=Truniger V, Lázaro JM, Salas M |title=Two positively charged residues of phi29 DNA polymerase, conserved in protein-primed DNA polymerases, are involved in stabilisation of the incoming nucleotide. |journal=J Mol Biol |volume=335 |issue=2 |pages=481-94 |year=2004 |pmid=14672657}}</ref>
<ref name="pmid12473453">{{cite journal |author=Rodríguez I, Lázaro JM, Salas M, de Vega M |title=phi29 DNA polymerase residue Phe128 of the highly conserved (S/T)Lx(2)h motif is required for a stable and functional interaction with the terminal protein. |journal=J Mol Biol |volume=325 |issue=1 |pages=85-97 |year=2003 |pmid=12473453}}</ref>
<ref name="pmid12083397">{{cite journal |author=Nelson JR, Cai YC, Giesler TL, Farchaus JW, Sundaram ST, Ortiz-Rivera M, Hosta LP, Hewitt PL, Mamone JA, Palaniappan C, Fuller CW |title=TempliPhi, phi29 DNA polymerase based rolling circle amplification of templates for DNA sequencing. |journal=Biotechniques |volume=Suppl |issue= |pages=44-7 |year=2002 |pmid=12083397}}</ref>
<ref name="pmid12054770">{{cite journal |author=Truniger V, Lázaro JM, Blanco L, Salas M |title=A highly conserved lysine residue in phi29 DNA polymerase is important for correct binding of the templating nucleotide during initiation of phi29 DNA replication. |journal=J Mol Biol |volume=318 |issue=1 |pages=83-96 |year=2002 |pmid=12054770}}</ref>
<ref name="pmid11917008">{{cite journal |author=Truniger V, Lázaro JM, Esteban FJ, Blanco L, Salas M |title=A positively charged residue of phi29 DNA polymerase, highly conserved in DNA polymerases from families A and B, is involved in binding the incoming nucleotide. |journal=Nucleic Acids Res |volume=30 |issue=7 |pages=1483-92 |year=2002 |pmid=11917008}}</ref>
<ref name="pmid11884636">{{cite journal |author=Eisenbrandt R, Lázaro JM, Salas M, de Vega M |title=Phi29 DNA polymerase residues Tyr59, His61 and Phe69 of the highly conserved ExoII motif are essential for interaction with the terminal protein. |journal=Nucleic Acids Res |volume=30 |issue=6 |pages=1379-86 |year=2002 |pmid=11884636}}</ref>
<ref name="pmid10523310">{{cite journal |author=Elías-Arnanz M, Salas M |title=Resolution of head-on collisions between the transcription machinery and bacteriophage phi29 DNA polymerase is dependent on RNA polymerase translocation. |journal=EMBO J |volume=18 |issue=20 |pages=5675-82 |year=1999 |pmid=10523310}}</ref>
<ref name="pmid10493855">{{cite journal |author=de Vega M, Blanco L, Salas M |title=Processive proofreading and the spatial relationship between polymerase and exonuclease active sites of bacteriophage phi29 DNA polymerase. |journal=J Mol Biol |volume=292 |issue=1 |pages=39-51 |year=1999 |pmid=10493855}}</ref>
<ref name="pmid10388570">{{cite journal |author=Bonnin A, Lázaro JM, Blanco L, Salas M |title=A single tyrosine prevents insertion of ribonucleotides in the eukaryotic-type phi29 DNA polymerase. |journal=J Mol Biol |volume=290 |issue=1 |pages=241-51 |year=1999 |pmid=10388570}}</ref>
<ref name="pmid9931249">{{cite journal |author=Truniger V, Blanco L, Salas M |title=Role of the "YxGG/A" motif of Phi29 DNA polymerase in protein-primed replication. |journal=J Mol Biol |volume=286 |issue=1 |pages=57-69 |year=1999 |pmid=9931249}}</ref>
<ref name="pmid9786901">{{cite journal |author=de Vega M, Blanco L, Salas M |title=phi29 DNA polymerase residue Ser122, a single-stranded DNA ligand for 3'-5' exonucleolysis, is required to interact with the terminal protein. |journal=J Biol Chem |volume=273 |issue=44 |pages=28966-77 |year=1998 |pmid=9786901}}</ref>
<ref name="pmid9784372">{{cite journal |author=Saturno J, Lázaro JM, Blanco L, Salas M |title=Role of the first aspartate residue of the "YxDTDS" motif of phi29 DNA polymerase as a metal ligand during both TP-primed and DNA-primed DNA synthesis. |journal=J Mol Biol |volume=283 |issue=3 |pages=633-42 |year=1998 |pmid=9784372}}</ref>
<ref name="pmid9723918">{{cite journal |author=Murthy V, Meijer WJ, Blanco L, Salas M |title=DNA polymerase template switching at specific sites on the phi29 genome causes the in vivo accumulation of subgenomic phi29 DNA molecules. |journal=Mol Microbiol |volume=29 |issue=3 |pages=787-98 |year=1998 |pmid=9723918}}</ref>
<ref name="pmid9705251">{{cite journal |author=Illana B, Zaballos A, Blanco L, Salas M |title=The RGD sequence in phage phi29 terminal protein is required for interaction with phi29 DNA polymerase. |journal=Virology |volume=248 |issue=1 |pages=12-9 |year=1998 |pmid=9705251}}</ref>
<ref name="pmid9642062">{{cite journal |author=de Vega M, Lázaro JM, Salas M, Blanco L |title=Mutational analysis of phi29 DNA polymerase residues acting as ssDNA ligands for 3'-5' exonucleolysis. |journal=J Mol Biol |volume=279 |issue=4 |pages=807-22 |year=1998 |pmid=9642062}}</ref>
<ref name="pmid8621470">{{cite journal |author=Blanco L, Salas M |title=Relating structure to function in phi29 DNA polymerase. |journal=J Biol Chem |volume=271 |issue=15 |pages=8509-12 |year=1996 |pmid=8621470}}</ref>
<ref name="pmid8605889">{{cite journal |author=de Vega M, Lazaro JM, Salas M, Blanco L |title=Primer-terminus stabilization at the 3'-5' exonuclease active site of phi29 DNA polymerase. Involvement of two amino acid residues highly conserved in proofreading DNA polymerases. |journal=EMBO J |volume=15 |issue=5 |pages=1182-92 |year=1996 |pmid=8605889}}</ref>
<ref name="pmid107317">{{cite journal |author=Sogo JM, Inciarte MR, Corral J, Viñuela E, Salas M |title=RNA polymerase binding sites and transcription map of the DNA of Bacillus subtilis phage phi29. |journal=J Mol Biol |volume=127 |issue=4 |pages=411-36 |year=1979 |pmid=107317}}</ref>
<ref name="pmid827446">{{cite journal |author=Inciarte MR, Viñuela E, Salas M |title=Transcription in vitro of phi29 DNA and EcoRI fragments by Bacillus subtilis RNA polymerase. |journal=Eur J Biochem |volume=71 |issue=1 |pages=77-83 |year=1976 |pmid=827446}}</ref>
<ref name="pmid4210355">{{cite journal |author=Jiménez F, Avila J, Viñuela E, Salas M |title=Initiation of the transcription of phi29 DNA by Bacillus subtilis RNA polymerase. |journal=Biochim Biophys Acta |volume=349 |issue=3 |pages=320-7 |year=1974 |pmid=4210355}}</ref>
</div>
{{reflist|2}}


[[Category:DNA replication]]
[[Category:DNA replication]]

Revision as of 14:30, 10 November 2010

Φ29 DNA polymerase is an enzyme from the bacteriophage Φ29. It is being increasingly used in molecular biology for multiple displacement DNA amplification procedures, and has a number of features that make it particularly suitable for this application.

Further reading

  1. ^ Linck L, Resch-Genger U (2010). "Identification of efficient fluorophores for the direct labeling of DNA via rolling circle amplification (RCA) polymerase φ29". Eur J Med Chem. PMID 20926164.
  2. ^ de Vega M, Lázaro JM, Mencía M, Blanco L, Salas M (2010). "Improvement of φ29 DNA polymerase amplification performance by fusion of DNA binding motifs". Proc Natl Acad Sci U S A. 107 (38): 16506–11. PMID 20823261.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  3. ^ Pérez-Arnaiz P, Lázaro JM, Salas M, de Vega M (2010). "phi29 DNA polymerase active site: role of residue Val250 as metal-dNTP complex ligand and in protein-primed initiation". J Mol Biol. 395 (2): 223–33. PMID 19883660.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  4. ^ Pérez-Arnaiz P, Lázaro JM, Salas M, de Vega M (2009). "Functional importance of bacteriophage phi29 DNA polymerase residue Tyr148 in primer-terminus stabilisation at the 3'-5' exonuclease active site". J Mol Biol. 391 (5): 797–807. PMID 19576228.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  5. ^ Johne R, Müller H, Rector A, van Ranst M, Stevens H (2009). "Rolling-circle amplification of viral DNA genomes using phi29 polymerase". Trends Microbiol. 17 (5): 205–11. PMID 19375325.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  6. ^ Alsmadi O, Alkayal F, Monies D, Meyer BF (2009). "Specific and complete human genome amplification with improved yield achieved by phi29 DNA polymerase and a novel primer at elevated temperature". BMC Res Notes. 2: 48. PMID 19309528.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  7. ^ Lagunavicius A, Merkiene E, Kiveryte Z, Savaneviciute A, Zimbaite-Ruskuliene V, Radzvilavicius T, Janulaitis A (2009). "Novel application of Phi29 DNA polymerase: RNA detection and analysis in vitro and in situ by target RNA-primed RCA". RNA. 15 (5): 765–71. PMID 19244362.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  8. ^ Rodríguez I, Lázaro JM, Salas M, de Vega M (2009). "Involvement of the TPR2 subdomain movement in the activities of phi29 DNA polymerase". Nucleic Acids Res. 37 (1): 193–203. PMID 19033368.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  9. ^ Sahu S, LaBean TH, Reif JH (2008). "A DNA nanotransport device powered by polymerase phi29". Nano Lett. 8 (11): 3870–8. PMID 18939810.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  10. ^ Xu Y, Gao S, Bruno JF, Luft BJ, Dunn JJ (2008). "Rapid detection and identification of a pathogen's DNA using Phi29 DNA polymerase". Biochem Biophys Res Commun. 375 (4): 522–5. PMID 18755142.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  11. ^ Kumar G, Garnova E, Reagin M, Vidali A (2008). "Improved multiple displacement amplification with phi29 DNA polymerase for genotyping of single human cells". Biotechniques. 44 (7): 879–90. PMID 18533898.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  12. ^ Salas M, Blanco L, Lázaro JM, de Vega M (2008). "The bacteriophage phi29 DNA polymerase". IUBMB Life. 60 (1): 82–5. PMID 18379997.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  13. ^ Silander K, Saarela J (2008). "Whole genome amplification with Phi29 DNA polymerase to enable genetic or genomic analysis of samples of low DNA yield". Methods Mol Biol. 439: 1–18. PMID 18370092.
  14. ^ Lagunavicius A, Kiveryte Z, Zimbaite-Ruskuliene V, Radzvilavicius T, Janulaitis A (2008). "Duality of polynucleotide substrates for Phi29 DNA polymerase: 3'-->5' RNase activity of the enzyme". RNA. 14 (3): 503–13. PMID 18230765.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  15. ^ Pérez-Arnaiz P, Longás E, Villar L, Lázaro JM, Salas M, de Vega M (2007). "Involvement of phage phi29 DNA polymerase and terminal protein subdomains in conferring specificity during initiation of protein-primed DNA replication". Nucleic Acids Res. 35 (21): 7061–73. PMID 17913744.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  16. ^ Berman AJ, Kamtekar S, Goodman JL, Lázaro JM, de Vega M, Blanco L, Salas M, Steitz TA (2007). "Structures of phi29 DNA polymerase complexed with substrate: the mechanism of translocation in B-family polymerases". EMBO J. 26 (14): 3494–505. PMID 17611604.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  17. ^ Knierim D, Maiss E (2007). "Application of Phi29 DNA polymerase in identification and full-length clone inoculation of tomato yellow leaf curl Thailand virus and tobacco leaf curl Thailand virus". Arch Virol. 152 (5): 941–54. PMID 17226067.
  18. ^ Owor BE, Shepherd DN, Taylor NJ, Edema R, Monjane AL, Thomson JA, Martin DP, Varsani A (2007). "Successful application of FTA Classic Card technology and use of bacteriophage phi29 DNA polymerase for large-scale field sampling and cloning of complete maize streak virus genomes". J Virol Methods. 140 (1–2): 100–5. PMID 17174409.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  19. ^ Sato M, Ohtsuka M, Ohmi Y (2004). "Repeated GenomiPhi, phi29 DNA polymerase-based rolling circle amplification, is useful for generation of large amounts of plasmid DNA". Nucleic Acids Symp Ser (Oxf) (48): 147–8. PMID 17150521.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  20. ^ Pérez-Arnaiz P, Lázaro JM, Salas M, de Vega M (2006). "Involvement of phi29 DNA polymerase thumb subdomain in the proper coordination of synthesis and degradation during DNA replication". Nucleic Acids Res. 34 (10): 3107–15. PMID 16757576.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  21. ^ Kamtekar S, Berman AJ, Wang J, Lázaro JM, de Vega M, Blanco L, Salas M, Steitz TA (2006). "The phi29 DNA polymerase:protein-primer structure suggests a model for the initiation to elongation transition". EMBO J. 25 (6): 1335–43. PMID 16511564.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  22. ^ Hutchison CA, Smith HO, Pfannkoch C, Venter JC (2005). "Cell-free cloning using phi29 DNA polymerase". Proc Natl Acad Sci U S A. 102 (48): 17332–6. PMID 16286637.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  23. ^ Sato M, Ohtsuka M, Ohmi Y (2005). "Usefulness of repeated GenomiPhi, a phi29 DNA polymerase-based rolling circle amplification kit, for generation of large amounts of plasmid DNA". Biomol Eng. 22 (4): 129–32. PMID 16023891.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  24. ^ Rodríguez I, Lázaro JM, Blanco L, Kamtekar S, Berman AJ, Wang J, Steitz TA, Salas M, de Vega M (2005). "A specific subdomain in phi29 DNA polymerase confers both processivity and strand-displacement capacity". Proc Natl Acad Sci U S A. 102 (18): 6407–12. PMID 15845765.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  25. ^ Truniger V, Bonnin A, Lázaro JM, de Vega M, Salas M (2005). "Involvement of the "linker" region between the exonuclease and polymerization domains of phi29 DNA polymerase in DNA and TP binding". Gene. 348: 89–99. PMID 15777661.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  26. ^ Umetani N, de Maat MF, Mori T, Takeuchi H, Hoon DS (2005). "Synthesis of universal unmethylated control DNA by nested whole genome amplification with phi29 DNA polymerase". Biochem Biophys Res Commun. 329 (1): 219–23. PMID 15721296.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  27. ^ Gadkar V, Rillig MC (2005). "Application of Phi29 DNA polymerase mediated whole genome amplification on single spores of arbuscular mycorrhizal (AM) fungi". FEMS Microbiol Lett. 242 (1): 65–71. PMID 15621421.
  28. ^ Kamtekar S, Berman AJ, Wang J, Lázaro JM, de Vega M, Blanco L, Salas M, Steitz TA (2004). "Insights into strand displacement and processivity from the crystal structure of the protein-primed DNA polymerase of bacteriophage phi29". Mol Cell. 16 (4): 609–18. PMID 15546620.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  29. ^ Adachi E, Shimamura K, Wakamatsu S, Kodama H (2004). "Amplification of plant genomic DNA by Phi29 DNA polymerase for use in physical mapping of the hypermethylated genomic region". Plant Cell Rep. 23 (3): 144–7. PMID 15168072.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  30. ^ Rodríguez I, Lázaro JM, Salas M, De Vega M (2004). "phi29 DNA polymerase-terminal protein interaction. Involvement of residues specifically conserved among protein-primed DNA polymerases". J Mol Biol. 337 (4): 829–41. PMID 15033354.{{cite journal}}: CS1 maint: multiple names: authors list (link)
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  33. ^ Truniger V, Lázaro JM, Salas M (2004). "Two positively charged residues of phi29 DNA polymerase, conserved in protein-primed DNA polymerases, are involved in stabilisation of the incoming nucleotide". J Mol Biol. 335 (2): 481–94. PMID 14672657.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  34. ^ Rodríguez I, Lázaro JM, Salas M, de Vega M (2003). "phi29 DNA polymerase residue Phe128 of the highly conserved (S/T)Lx(2)h motif is required for a stable and functional interaction with the terminal protein". J Mol Biol. 325 (1): 85–97. PMID 12473453.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  35. ^ Nelson JR, Cai YC, Giesler TL, Farchaus JW, Sundaram ST, Ortiz-Rivera M, Hosta LP, Hewitt PL, Mamone JA, Palaniappan C, Fuller CW (2002). "TempliPhi, phi29 DNA polymerase based rolling circle amplification of templates for DNA sequencing". Biotechniques. Suppl: 44–7. PMID 12083397.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  36. ^ Truniger V, Lázaro JM, Blanco L, Salas M (2002). "A highly conserved lysine residue in phi29 DNA polymerase is important for correct binding of the templating nucleotide during initiation of phi29 DNA replication". J Mol Biol. 318 (1): 83–96. PMID 12054770.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  37. ^ Truniger V, Lázaro JM, Esteban FJ, Blanco L, Salas M (2002). "A positively charged residue of phi29 DNA polymerase, highly conserved in DNA polymerases from families A and B, is involved in binding the incoming nucleotide". Nucleic Acids Res. 30 (7): 1483–92. PMID 11917008.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  38. ^ Eisenbrandt R, Lázaro JM, Salas M, de Vega M (2002). "Phi29 DNA polymerase residues Tyr59, His61 and Phe69 of the highly conserved ExoII motif are essential for interaction with the terminal protein". Nucleic Acids Res. 30 (6): 1379–86. PMID 11884636.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  39. ^ Elías-Arnanz M, Salas M (1999). "Resolution of head-on collisions between the transcription machinery and bacteriophage phi29 DNA polymerase is dependent on RNA polymerase translocation". EMBO J. 18 (20): 5675–82. PMID 10523310.
  40. ^ de Vega M, Blanco L, Salas M (1999). "Processive proofreading and the spatial relationship between polymerase and exonuclease active sites of bacteriophage phi29 DNA polymerase". J Mol Biol. 292 (1): 39–51. PMID 10493855.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  41. ^ Bonnin A, Lázaro JM, Blanco L, Salas M (1999). "A single tyrosine prevents insertion of ribonucleotides in the eukaryotic-type phi29 DNA polymerase". J Mol Biol. 290 (1): 241–51. PMID 10388570.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  42. ^ Truniger V, Blanco L, Salas M (1999). "Role of the "YxGG/A" motif of Phi29 DNA polymerase in protein-primed replication". J Mol Biol. 286 (1): 57–69. PMID 9931249.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  43. ^ de Vega M, Blanco L, Salas M (1998). "phi29 DNA polymerase residue Ser122, a single-stranded DNA ligand for 3'-5' exonucleolysis, is required to interact with the terminal protein". J Biol Chem. 273 (44): 28966–77. PMID 9786901.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  44. ^ Saturno J, Lázaro JM, Blanco L, Salas M (1998). "Role of the first aspartate residue of the "YxDTDS" motif of phi29 DNA polymerase as a metal ligand during both TP-primed and DNA-primed DNA synthesis". J Mol Biol. 283 (3): 633–42. PMID 9784372.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  45. ^ Murthy V, Meijer WJ, Blanco L, Salas M (1998). "DNA polymerase template switching at specific sites on the phi29 genome causes the in vivo accumulation of subgenomic phi29 DNA molecules". Mol Microbiol. 29 (3): 787–98. PMID 9723918.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  46. ^ Illana B, Zaballos A, Blanco L, Salas M (1998). "The RGD sequence in phage phi29 terminal protein is required for interaction with phi29 DNA polymerase". Virology. 248 (1): 12–9. PMID 9705251.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  47. ^ de Vega M, Lázaro JM, Salas M, Blanco L (1998). "Mutational analysis of phi29 DNA polymerase residues acting as ssDNA ligands for 3'-5' exonucleolysis". J Mol Biol. 279 (4): 807–22. PMID 9642062.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  48. ^ Blanco L, Salas M (1996). "Relating structure to function in phi29 DNA polymerase". J Biol Chem. 271 (15): 8509–12. PMID 8621470.
  49. ^ de Vega M, Lazaro JM, Salas M, Blanco L (1996). "Primer-terminus stabilization at the 3'-5' exonuclease active site of phi29 DNA polymerase. Involvement of two amino acid residues highly conserved in proofreading DNA polymerases". EMBO J. 15 (5): 1182–92. PMID 8605889.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  50. ^ Sogo JM, Inciarte MR, Corral J, Viñuela E, Salas M (1979). "RNA polymerase binding sites and transcription map of the DNA of Bacillus subtilis phage phi29". J Mol Biol. 127 (4): 411–36. PMID 107317.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  51. ^ Inciarte MR, Viñuela E, Salas M (1976). "Transcription in vitro of phi29 DNA and EcoRI fragments by Bacillus subtilis RNA polymerase". Eur J Biochem. 71 (1): 77–83. PMID 827446.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  52. ^ Jiménez F, Avila J, Viñuela E, Salas M (1974). "Initiation of the transcription of phi29 DNA by Bacillus subtilis RNA polymerase". Biochim Biophys Acta. 349 (3): 320–7. PMID 4210355.{{cite journal}}: CS1 maint: multiple names: authors list (link)
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