Read (biology): Difference between revisions
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In biology, in the contexts of [[genomics]], [[transcriptomics]], [[DNA_sequencing]], and [[RNA-Seq]], a "read" is a short sequence of DNA, obtained from a library of DNA fragments. <ref name = Roach>{{cite journal|last1=Roach|first1=JC|last2=Boysen|first2=C|last3=Wang|first3=K|last4=Hood|first4=L|title=Pairwise end sequencing: a unified approach to genomic mapping and sequencing.|journal=Genomics|date=20 March 1995|volume=26|issue=2|pages=345-53|pmid=7601461}}</ref>. In [[DNA_sequencing]], a library of DNA fragments is prepared. For the RNA-Seq methodology, RNA is converted in DNA ([[reverse transcription]]), fragmented and sequenced based on [[High-throughput sequencing]] technologies; the final result is millions of reads. The length of a read is typically 25-400 base pairs long, depending on the sequencing technology used <ref name = Liu>{{cite journal|last1=Liu|first1=Lin|last2=Li|first2=Yinhu|last3=Li|first3=Siliang|last4=Hu|first4=Ni|last5=He|first5=Yimin|last6=Pong|first6=Ray|last7=Lin|first7=Danni|last8=Lu|first8=Lihua|last9=Law|first9=Maggie|title=Comparison of Next-Generation Sequencing Systems|journal=Journal of Biomedicine and Biotechnology|date=2012|volume=2012|pages=1–11|doi=10.1155/2012/251364}}</ref>. Read length is a factor which can affect the results of biological studies. <ref name = Sagar>{{cite journal|last1=Chhangawala|first1=Sagar|last2=Rudy|first2=Gabe|last3=Mason|first3=Christopher E.|last4=Rosenfeld|first4=Jeffrey A.|title=The impact of read length on quantification of differentially expressed genes and splice junction detection|journal=Genome Biology|date=23 June 2015|volume=16|issue=1|doi=10.1186/s13059-015-0697-y}}</ref>. |
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In biology, [[genomics]], [[transcriptomics]], DNA-Seq or [[RNA-Seq]] context, "read" means a short sequence of dna, typically 25-400 base pairs long. Basically, reads are raw sequences that come off a sequencing machine. In the RNA-Seq methodology, RNA is converted in DNA ([[reverse transcription]]), fragmented and sequenced based on [[High-throughput sequencing]] technologies; the final result is millions of reads. |
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[[Category:DNA]] |
[[Category:DNA]] |
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== References == |
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Revision as of 12:11, 23 July 2017
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In biology, in the contexts of genomics, transcriptomics, DNA_sequencing, and RNA-Seq, a "read" is a short sequence of DNA, obtained from a library of DNA fragments. [1]. In DNA_sequencing, a library of DNA fragments is prepared. For the RNA-Seq methodology, RNA is converted in DNA (reverse transcription), fragmented and sequenced based on High-throughput sequencing technologies; the final result is millions of reads. The length of a read is typically 25-400 base pairs long, depending on the sequencing technology used [2]. Read length is a factor which can affect the results of biological studies. [3].
References
- ^ Roach, JC; Boysen, C; Wang, K; Hood, L (20 March 1995). "Pairwise end sequencing: a unified approach to genomic mapping and sequencing". Genomics. 26 (2): 345–53. PMID 7601461.
- ^ Liu, Lin; Li, Yinhu; Li, Siliang; Hu, Ni; He, Yimin; Pong, Ray; Lin, Danni; Lu, Lihua; Law, Maggie (2012). "Comparison of Next-Generation Sequencing Systems". Journal of Biomedicine and Biotechnology. 2012: 1–11. doi:10.1155/2012/251364.
{{cite journal}}: CS1 maint: unflagged free DOI (link) - ^ Chhangawala, Sagar; Rudy, Gabe; Mason, Christopher E.; Rosenfeld, Jeffrey A. (23 June 2015). "The impact of read length on quantification of differentially expressed genes and splice junction detection". Genome Biology. 16 (1). doi:10.1186/s13059-015-0697-y.
{{cite journal}}: CS1 maint: unflagged free DOI (link)