Schneider 2 cells: Difference between revisions
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Several media have been developed for culturing insect cell lines with many of them suitable for culturing S2 cells. The S2 cells have been shown to grow up to 5.1×10<sup>7</sup> cells/ml in serum free medium<ref>{{Cite journal|last=Pamboukian|first=Marilena Martins|last2=Jorge|first2=Soraia Athie Calil|last3=Santos|first3=Mariza Gerdulo|last4=Yokomizo|first4=Adriana Yurie|last5=Pereira|first5=Carlos Augusto|last6=Tonso|first6=Aldo|date=2008-02-27|title=Insect cells respiratory activity in bioreactor|url=http://link.springer.com/article/10.1007/s10616-007-9118-8|journal=Cytotechnology|language=en|volume=57|issue=1|pages=37–44|doi=10.1007/s10616-007-9118-8|issn=0920-9069|pmc=2553646|pmid=19003170}}</ref> and above 10<sup>7</sup> cells/ml in basal media such as that used in Schneider's experiments.<ref>{{Cite journal|last=Moraes|first=Ângela M.|last2=Jorge|first2=Soraia A. C.|last3=Astray|first3=Renato M.|last4=Suazo|first4=Claudio A. T.|last5=Calderón Riquelme|first5=Camilo E.|last6=Augusto|first6=Elisabeth F. P.|last7=Tonso|first7=Aldo|last8=Pamboukian|first8=Marilena M.|last9=Piccoli|first9=Rosane A. M.|date=2012-05-01|title=Drosophila melanogaster S2 cells for expression of heterologous genes: From gene cloning to bioprocess development|url=http://www.sciencedirect.com/science/article/pii/S073497501100190X|journal=Biotechnology Advances|volume=30|issue=3|pages=613–628|doi=10.1016/j.biotechadv.2011.10.009}}</ref> [[ExpreS2ion Biotechnologies|ExpreS<sup>2</sup>ion Biotechnologies]] have shown a cell concentration of up 7.0x10<sup>7</sup> cell/ml.<ref>{{Cite journal|last=Dyring|first=Charlotte|title=Optimising the Drosophila S2 Expression System for Production of Therapeutic Vaccines|url=http://www.bioprocessingjournal.com/index.php/article-downloads/405-j102-optimising-the-drosophila-s2-expression-system-for-production-of-therapeutic-vaccines|journal=BioProcessing Journal|volume=10|issue=2|pages=28–35|doi=10.12665/j102.dyring}}</ref> |
Several media have been developed for culturing insect cell lines with many of them suitable for culturing S2 cells. The S2 cells have been shown to grow up to 5.1×10<sup>7</sup> cells/ml in serum free medium<ref>{{Cite journal|last=Pamboukian|first=Marilena Martins|last2=Jorge|first2=Soraia Athie Calil|last3=Santos|first3=Mariza Gerdulo|last4=Yokomizo|first4=Adriana Yurie|last5=Pereira|first5=Carlos Augusto|last6=Tonso|first6=Aldo|date=2008-02-27|title=Insect cells respiratory activity in bioreactor|url=http://link.springer.com/article/10.1007/s10616-007-9118-8|journal=Cytotechnology|language=en|volume=57|issue=1|pages=37–44|doi=10.1007/s10616-007-9118-8|issn=0920-9069|pmc=2553646|pmid=19003170}}</ref> and above 10<sup>7</sup> cells/ml in basal media such as that used in Schneider's experiments.<ref>{{Cite journal|last=Moraes|first=Ângela M.|last2=Jorge|first2=Soraia A. C.|last3=Astray|first3=Renato M.|last4=Suazo|first4=Claudio A. T.|last5=Calderón Riquelme|first5=Camilo E.|last6=Augusto|first6=Elisabeth F. P.|last7=Tonso|first7=Aldo|last8=Pamboukian|first8=Marilena M.|last9=Piccoli|first9=Rosane A. M.|date=2012-05-01|title=Drosophila melanogaster S2 cells for expression of heterologous genes: From gene cloning to bioprocess development|url=http://www.sciencedirect.com/science/article/pii/S073497501100190X|journal=Biotechnology Advances|volume=30|issue=3|pages=613–628|doi=10.1016/j.biotechadv.2011.10.009}}</ref> [[ExpreS2ion Biotechnologies|ExpreS<sup>2</sup>ion Biotechnologies]] have shown a cell concentration of up 7.0x10<sup>7</sup> cell/ml.<ref>{{Cite journal|last=Dyring|first=Charlotte|title=Optimising the Drosophila S2 Expression System for Production of Therapeutic Vaccines|url=http://www.bioprocessingjournal.com/index.php/article-downloads/405-j102-optimising-the-drosophila-s2-expression-system-for-production-of-therapeutic-vaccines|journal=BioProcessing Journal|volume=10|issue=2|pages=28–35|doi=10.12665/j102.dyring}}</ref> |
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S2 cells are often used for expression of heterelogous proteins and can be used for large-scale production of proteins. Additionally, the cells can be easily transiently transfected with several plasmids at once to study protein interactions.<ref>{{Cite journal|last=Fasbender|first=Frank|last2=Claus|first2=Maren|last3=Wingert|first3=Sabine|last4=Sandusky|first4=Mina|last5=Watzl|first5=Carsten|date=2017-07-07|title=Differential Requirements for Src-Family Kinases in SYK or ZAP70-Mediated SLP-76 Phosphorylation in Lymphocytes|url=http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5500614/|journal=Frontiers in Immunology|volume=8|doi=10.3389/fimmu.2017.00789|issn=1664-3224|pmc=PMC5500614|pmid=28736554}}</ref> |
S2 cells are often used for expression of heterelogous proteins and can be used for large-scale production of proteins. Additionally, the cells can be easily transiently transfected with several plasmids at once to study protein interactions.<ref>{{Cite journal|last=Fasbender|first=Frank|last2=Claus|first2=Maren|last3=Wingert|first3=Sabine|last4=Sandusky|first4=Mina|last5=Watzl|first5=Carsten|date=2017-07-07|title=Differential Requirements for Src-Family Kinases in SYK or ZAP70-Mediated SLP-76 Phosphorylation in Lymphocytes|url=http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5500614/|journal=Frontiers in Immunology|volume=8|doi=10.3389/fimmu.2017.00789|issn=1664-3224|pmc=PMC5500614|pmid=28736554}}</ref><ref>{{Cite journal|last=Yang|first=Jianying|last2=Reth|first2=Michael|date=2012|title=Drosophila S2 Schneider cells: a useful tool for rebuilding and redesigning approaches in synthetic biology|url=https://www.ncbi.nlm.nih.gov/pubmed/22083752|journal=Methods in Molecular Biology (Clifton, N.J.)|volume=813|pages=331–341|doi=10.1007/978-1-61779-412-4_20|issn=1940-6029|pmid=22083752}}</ref> |
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== References == |
== References == |
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Revision as of 19:29, 5 August 2017
Schneider 2 cells, usually abbreviated as S2 cells, are one of the most commonly used Drosophila melanogaster cell lines. S2 cells were derived from a primary culture of late stage (20–24 hours old) Drosophila melanogaster embryos,[1] likely from a macrophage-like lineage.
S2 cells can be grown at room temperature both as a semi-adherent monolayer or in suspension, and they can be grown in the absence of serum.[2]
Several media have been developed for culturing insect cell lines with many of them suitable for culturing S2 cells. The S2 cells have been shown to grow up to 5.1×107 cells/ml in serum free medium[3] and above 107 cells/ml in basal media such as that used in Schneider's experiments.[4] ExpreS2ion Biotechnologies have shown a cell concentration of up 7.0x107 cell/ml.[5]
S2 cells are often used for expression of heterelogous proteins and can be used for large-scale production of proteins. Additionally, the cells can be easily transiently transfected with several plasmids at once to study protein interactions.[6][7]
References
- ^ Schneider I (1972). "Cell Lines Derived from Late Embryonic Stages of Drosophila melanogaster". J. Embryol. Exp. Morph. 27: 363–365.
- ^ Invitrogen (05/02/2002). Drosophila Schneider 2 (S2) Cells (PDF). Version F. Invitrogen. 28-0172. Retrieved 2012-02-25.
{{cite book}}: Check date values in:|date=(help); Cite has empty unknown parameter:|sectionurl=(help) - ^ Pamboukian, Marilena Martins; Jorge, Soraia Athie Calil; Santos, Mariza Gerdulo; Yokomizo, Adriana Yurie; Pereira, Carlos Augusto; Tonso, Aldo (2008-02-27). "Insect cells respiratory activity in bioreactor". Cytotechnology. 57 (1): 37–44. doi:10.1007/s10616-007-9118-8. ISSN 0920-9069. PMC 2553646. PMID 19003170.
- ^ Moraes, Ângela M.; Jorge, Soraia A. C.; Astray, Renato M.; Suazo, Claudio A. T.; Calderón Riquelme, Camilo E.; Augusto, Elisabeth F. P.; Tonso, Aldo; Pamboukian, Marilena M.; Piccoli, Rosane A. M. (2012-05-01). "Drosophila melanogaster S2 cells for expression of heterologous genes: From gene cloning to bioprocess development". Biotechnology Advances. 30 (3): 613–628. doi:10.1016/j.biotechadv.2011.10.009.
- ^ Dyring, Charlotte. "Optimising the Drosophila S2 Expression System for Production of Therapeutic Vaccines". BioProcessing Journal. 10 (2): 28–35. doi:10.12665/j102.dyring.
- ^ Fasbender, Frank; Claus, Maren; Wingert, Sabine; Sandusky, Mina; Watzl, Carsten (2017-07-07). "Differential Requirements for Src-Family Kinases in SYK or ZAP70-Mediated SLP-76 Phosphorylation in Lymphocytes". Frontiers in Immunology. 8. doi:10.3389/fimmu.2017.00789. ISSN 1664-3224. PMC 5500614. PMID 28736554.
{{cite journal}}: CS1 maint: PMC format (link) CS1 maint: unflagged free DOI (link) - ^ Yang, Jianying; Reth, Michael (2012). "Drosophila S2 Schneider cells: a useful tool for rebuilding and redesigning approaches in synthetic biology". Methods in Molecular Biology (Clifton, N.J.). 813: 331–341. doi:10.1007/978-1-61779-412-4_20. ISSN 1940-6029. PMID 22083752.