Diff-Quik: Difference between revisions
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Diff-Quik is utilized on material which is ''air-dried'' prior to alcohol [[Fixation_(histology)|fixation]] (rather than immersed immediately i.e. "wet-fixed").<ref name="Lester2019"></ref> |
Diff-Quik is utilized on material which is ''air-dried'' prior to alcohol [[Fixation_(histology)|fixation]] (rather than immersed immediately i.e. "wet-fixed").<ref name="Lester2019"></ref> |
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The primary use of Romanowsky-type stains is for [[cytoplasm|cytoplasmic detail]], such as intracytoplasmic [[mucins]], fat droplets and neurosecretory granules. Extracellular substances, such as free mucin, colloid, ground substance, etc., are also easily stained, and appear metachromatic. Microbiologic agents, such as bacteria and fungi, also appear more easily in Diff-Quik. |
The primary use of Romanowsky-type stains is for [[cytoplasm|cytoplasmic detail]<ref name="FinnPeterson2004">{{cite book|author1=William G. Finn|author2=LoAnn C. Peterson|title=Hematopathology in Oncology|url=https://books.google.com/books?id=qi9c1xbvPyoC&pg=PA184|date=31 May 2004|publisher=Springer Science & Business Media|isbn=978-1-4020-7919-1|page=184|chapter=Role of fine needle aspiration in lymphoma}}</ref>], such as intracytoplasmic [[mucins]], fat droplets and neurosecretory granules. Extracellular substances, such as free mucin, colloid, ground substance, etc., are also easily stained, and appear metachromatic. Microbiologic agents, such as bacteria and fungi, also appear more easily in Diff-Quik. |
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<ref name="Demay"></ref> |
<ref name="Demay"></ref> |
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Revision as of 03:20, 29 June 2019
 | This article needs additional citations for verification. (November 2006) |
Diff-Quik is a commercial Romanowsky stain variant, commonly used in histological staining to rapidly stain and differentiate a variety of smears, commonly blood films and cytopathological smears, including those of fine needle aspirates and touch preparations.[1][2] The Diff-Quik procedure is based on a modification of the Wright Giemsa stain pioneered by Bernard Witlin in 1970, and has advantages over the routine Wright-Giemsa staining technique in that it reduces the 4-minute process into a simplified 15-second operation and allows for selective increased eosinophilic or basophilic staining depending upon the time the smear is left in the staining solutions.[3]
Diff-Quik is utilized on material which is air-dried prior to alcohol fixation (rather than immersed immediately i.e. "wet-fixed").[2]
The primary use of Romanowsky-type stains is for [[cytoplasm|cytoplasmic detail][4]], such as intracytoplasmic mucins, fat droplets and neurosecretory granules. Extracellular substances, such as free mucin, colloid, ground substance, etc., are also easily stained, and appear metachromatic. Microbiologic agents, such as bacteria and fungi, also appear more easily in Diff-Quik. [3]
There are generic brands of such stain, and the trade name is sometimes used loosely to refer to any such stain (much as "Coke" or "Band-Aid" are sometimes used imprecisely). The misspelling "Diff-Quick" is commonly encountered.
Components
The Diff-Quik stain consists of 3 solutions:
- Diff-Quik fixative reagent
- Diff-Quik solution I (eosinophilic)
- Diff-Quik solution II (basophilic)
- Thiazine dye
- pH buffer
Results
| Structure | Colour |
|---|---|
| Erythrocytes | Pink/yellowish red |
| Platelets | Violet/purple granules |
| Neutrophils | Blue nucleus, pink cytoplasm, violet granules |
| Eosinophils | Blue nucleus, blue cytoplasm, red granules |
| Basophils | Purple/dark blue nucleus, violet granules |
| Monocyte | Violet nucleus, light blue cytoplasm |
| Bacteria | Blue |
| Spermatozoa | Pale blue in the acrosomal region and dark blue in the post-acrosomal region[5] |
Alternatives
References
- ^ Silverman JF, Frable WJ (1990). "The use of the diff-quik stain in the immediate interpretation of fine-needle aspiration biopsies". Diagn Cytopathol. 6 (5): 366–9. PMID 1705500.
- ^ a b Susan C. Lester (10 May 2019). "Section 2: Methods - Slide preparation". In 2nd (ed.). Diagnostic Pathology: Intraoperative Consultation E-Book. Elsevier Health Sciences. p. 69. ISBN 978-0-323-57020-6.
{{cite book}}: CS1 maint: numeric names: editors list (link) - ^ a b Demay: The Art and Science of Cytopathology
- ^ William G. Finn; LoAnn C. Peterson (31 May 2004). "Role of fine needle aspiration in lymphoma". Hematopathology in Oncology. Springer Science & Business Media. p. 184. ISBN 978-1-4020-7919-1.
- ^ WHO laboratory manual for the examination and processing of human semen - 5th ed. 2010