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Maclean usually performed a live routine or routines with Glaze in front of a studio audience of children and a filmed insert with Glaze, in the style of a silent comedy film. Live routines would almost always work in the 'joke' where an exasperated Glaze would exclaim 'Maclean!' to which Maclean would answer 'Yes, I had a bath this morning!'. Also notable was that when responding to Glaze's exasperation, Maclean would regularly give an alliterative reply, such as "Don't get your knickers in a knot" or "Don't get your tights in a twist", the combination of which ("Don't get your knickers in a twist") has passed into popular vernacular.
Early in his career, Maclean was a comedy compère of the BBC Television Series The Black and White Minstrel Show. He also appeared in Crossroads and Carry On Columbus. A practising Roman Catholic, from 1990 Maclean presented BBC Radio 2's religious show Good Morning Sunday, until he was replaced by Aled Jones in 2006.
He hosted the panel games The Clever Dick-Athlon (1988–90), First Letter First (1993) and Are You Sitting Comfortably (1993–96), all for Radio 2. He also toured in the play There's No Place Like a Home with Gorden Kaye. In 2009, he claimed that the BBC is keen on programmes which attack churches and that there is a wider secularist campaign to get rid of Christianity.
- Comedian Don Maclean made Papal Knight, bbc.co.uk; accessed 27 May 2012.
- "BBC is anti-Christian and pro-Muslim says ex host", christian.org.uk; accessed 21 May 2015.