Immunomagnetic separation
 |
This article may need to be wikified to meet Wikipedia's quality standards. Please help by adding relevant internal links, or by improving the article's layout. (April 2010)
Click [show] on right for more details.
No reason has been cited for the Wikify tag on this article.
|
Immunomagnetic separation (IMS) is a laboratory tool that can efficiently isolate cells out of body fluid or cultured cells. It can also be used as a method of quantifying the pathogenicity of food, blood or feces. DNA analysis have supported the combined use of both this technique and Polymerase Chain Reaction (PCR)[1]. Another laboratory separation tool is the affinity magnetic separation (AMS), which is more suitable for the isolation of prokaryotic cells[2].
[edit] Technique
Antibodies coating paramagnetic beads will bind to antigens present on the surface of cells thus capturing the cells and facilitate the concentration of these bead-attached cells. The concentration process is created by a magnet placed on the side of the test tube bringing the beads to it.
[edit] References
- ^ Engstrand, L. and Enroth, H., Journal of Clinical microbiology, vol.33, no.8, August 1995, p. 2162-2165.
- ^ Affinity magnetic separation of Listeria spp and Escherichia coli O157 (Bacteria Capture Kit)
