Since 1963 he has published sketches and cartoons for Cuban magazines and newspapers. He was the creator, in 1970, of Elpidio Valdés, a cartoon character (and series) with more than sixty shorts and two feature-length movies. Padrón received international attention Vampires in Havana, an adult-oriented comedy feature that gathered a cult following abroad.
Padrón has directed six animated feature films in total, along with many shorts, especially those with Elpidio Valdés as the main characher; and the one-minute comedy sketches for adults "Filminutos".
In 2008, Padrón was selected as the recipient of Cuba's National Film Award.