Klose was born (as Frida Klose) and died in Berlin. She lost her father early in life and had to earn her living as a secretary, until a colleague recommended her to the Klindworth-Scharwenka conservatory, where she got a thorough musical education.
Klose made her début in 1926 at Ulm theatre in a supporting role of Emmerich Kalman's operetta Countess Maritza. Her next role was Azucena in Giuseppe Verdi's Il trovatore. For the next three years Klose sang in Ulm and Kassel, before joining the Nationaltheater Mannheim in 1929.
In addition she was a celebrated guest e. g. at Vienna State Opera, the Royal Opera House Covent Garden, La Scala in Milan, Sächsische Staatsoper Dresden, Nationaltheater München, Hamburgische Staatsoper, Teatro Colón in Buenos Aires, La Monnaie in Brussels, in San Francisco, and Los Angeles.
From 1936 to 1942, Klose regularly sang at the Bayreuth Festival every summer, where she became popular especially in the role of Brangäne in Tristan und Isolde. She also sang at the Richard Wagner-Festival in Sopot (1935) and the Salzburg Festival (1949 and 1955).
When the great Wagner soprano Frida Leider gave her farewell concert in Berlin in 1946, Klose was her partner on stage.
In 1968, she died suddenly at the age of 69.
During the 1930s and 1940s, Klose was considered the leading German mezzo, internationally sought after especially as Wagner-singer. In addition, she sang a lot of Verdi and Richard Strauss and was famous as Bach- and Lied-singer.
- Branscombe, Peter (1992), 'Klose, Margarete' in The New Grove Dictionary of Opera, ed. Stanley Sadie (London) ISBN 0-333-73432-7
- Seeger, Horst; Das große Lexikon der Oper, Herrsching, 1985, ISBN 3-88199-243-X
- Vieten, Jacob: Margarete Klose. [Discography, pictures, documents]. in: Stimmen die um die Welt gingen. Vol. 74. Münster (Germany) 2003
- Warrack, John and West, Ewan (1992), The Oxford Dictionary of Opera, 782 pages, ISBN 0-19-869164-5