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Ne'ila, (lit. locking) the concluding service, is a special Jewish prayer service that is held only on Yom Kippur. It is the time when final prayers of repentance are recited at the closing of Yom Kippur. Ne'ila consists of Ashrei, Uva L'Tzion (both of which are postponed from Mincha when they are normally recited), Amidah with Selichot and an abbreviated Vidui, and Avinu Malkeinu. Neilah marks the fifth Amidah of the Day of Atonement, the only such occasion in the Hebrew calendar in which there are so many services. The shofar is blown at the end of Ne'ila. During the leader's repetition of the Ne'ila Amidah, the ark (Aron Kodesh or Hechal) remains open, and it is traditional to stand throughout the service.

The service can also conclude with a Havdalah ceremony, similar to that concluding Sabbath. Yom Kippur is ended in the same style as Sabbath: separating the holy from the everyday.Neila is also performed on Rosh Hashana the Jewish New year.