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Nikolayev/Nikolaev (masculine) or Nikolayeva (feminine) may refer to:
- Nikolayev (surname) (Nikolayeva)
- Nikolayev, Russified spelling of Mykolaiv, the name of several places in Ukraine
- Nikolayev (crater), a Moon crater
- Nikolayevka (disambiguation)
- Nikolayevsk (disambiguation)
- Nikolayevsky (disambiguation)
- Nikolaevo, a town in Stara Zagora Province, Bulgaria
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