Salem Presbyterian Church is a historic church in Limestone, Tennessee, affiliated with the Holston Presbytery and Presbyterian Church (USA).
The congregation was formed in 1780 under the leadership of Reverend Samuel Doak. It was the first Presbyterian church established in the area that was later to become the state of Tennessee.
Construction of the church's current building began in 1894. Its sanctuary has several large stained glass windows.
The building was listed on the National Register of Historic Places in 1992.