Born in Chandigarh, Sameksha Singh is an Electronic Engineer and she made her debut in movies with Telugu film 143 directed by Telugu movie director Puri Jaggannath. Her first film in Tamil was Arinthum Ariyamalum by Tamil film director Vishnu Vardhan. won several awards for the movie Arinthum Ariyalmalum including the award for the best Debut Actress in Tamil industry. After making her debut in Tamil and Telugu movies she finished more than 15 movies in Tamil and Telugu.
She was also on the show "Zaara Pyaar Ki Saugat" which was airing on the channel Sahara One.
She appeared as a model for univercell mobile.
She appeared as a model for many print commercials for reputed brands like Kit-Kat, Monte Carlo, American Express Card, Amway, World Gold Council, Mother Dairy, Havell’s switch and T.V commercial’s like Johnson's baby prickly heat powder, Hyundai Xing, Sunsilk and Metro tyres, Univercell mobile. Samiksha made her debut in small screen with the T.V soap Zaara where she played the lead role as Sonia Kapoor on Sahara One. Samiksha was also seen playing the role of Nilanjana Roy in the popular daily soap Yahan Main Ghar Ghar Kheli on Zee TV. She also played the role of the much loved dead wife of the male protogonist ACP Arjun Suryakanth Raute(Shaleen Malhotra), Roshni in Action crime series Arjun (TV series) airing on Star Plus.