Selenite broth
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Selenite broth is used as a selective medium for the isolation of Salmonella species. This medium must not be autoclaved. Once prepared it is steamed at 100°C for 30 minutes. There should be a very slight red precipitate. To minimise the risk of teratogenicity to workers, sodium selenite must be added separately to the medium. It has a pH of approximately 7.1.
Selenite broth contains:
| Peptone | 5g/l |
| Mannitol | 4g/l |
| Di-sodium hydrogen phosphate | 9.5g/l |
| Sodium di-hydrogen phosphate | 10g/l |
| Sodium selenite (NaHSeO3) | 4g/l |
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