Spin trapping
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Spin trapping is an analytical technique employed in the detection and identification of short-lived free radicals. Spin trapping involves the addition of radical to a nitrone spin trap resulting in the formation of a spin adduct, a nitroxide-based persistent radical, that can be detected using electron paramagnetic resonance (EPR) spectroscopy. The spin adduct usually yields a distinctive EPR spectrum characteristic of a particular free radical that is trapped. The identity of the radical can be inferred based from the EPR spectral profile of their respective spin adducts such as the g value, but most importantly, the hyperfine-coupling constants of relevant nuclei.
The most commonly used spin traps are alpha-phenyl N-tertiary-butyl nitrone (PBN) and 5,5-dimethyl-pyrroline N-oxide (DMPO).
The use of radical-addition reactions to detect short-lived radicals was first proposed by E. G. Janzen in 1965.
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