Talk:Acid guanidinium thiocyanate-phenol-chloroform extraction

	This article is within the scope of WikiProject Molecular Biology, a collaborative effort to improve the coverage of Molecular Biology on Wikipedia. If you would like to participate, please visit the project page, where you can join the discussion and see a list of open tasks.Molecular BiologyWikipedia:WikiProject Molecular BiologyTemplate:WikiProject Molecular BiologyMolecular Biology articles
???	This article has not yet received a rating on the importance scale.
	This article is supported by the Molecular and Cell Biology task force (assessed as Low-importance).

Comment[edit]

I am not a fan of wiki's policy on how-tos but this is against the rules, so I copied it here before someone deletes it. read it here (hidden: go to edit or raw mode to retrieve) [1]

About the merge I am not sure about the direction --Squidonius (talk) 13:49 & 13:53, 18 April 2008 --Squidonius (talk) 14:28, 22 April 2008 (UTC)(UTC)[reply]

Merged as most editors here are theory people and not methods people. --Squidonius (talk) 23:54, 7 May 2008 (UTC)[reply]

- This article is wrong on several fronts. It's entitled phenol-chloroform_extraction, therefore should present that alone (or have separate section(s) or articles if necessary) for phenol/chloroform extraction, acid-phenol/chloroform extraction, choroform/iaa washing, salt/ethanol(or IPA) precipitation etc.... and yes include

guanidinium-cl/phenol/chloroform extraction too ....but basic phenol/chloroform-extraction as commonly referred to wouldn't include the guanidine as well!! ** — Preceding unsigned comment added by 62.6.248.170 (talk) 11:50, 10 November 2011 (UTC)[reply]

This article totally ignores the use of PCIA extraction for DNA purification, where it is frequently indispensable for final preparation for transfection or other procedures sensitive to protein contamination. Also some parts of the article are not consistent with what I has been explained to me about the way the procedure works (eg. I've been told that proteins will crash out at the interphase due to disruption of phenolic rings in the protein, not dissolved in the organic layer). I'll verify some of this stuff and I will probably modify the article to some extent. Vladdybhs (talk) 15:41, 6 August 2008 (UTC)[reply]

I mostly agree: the 'How it works' setcion describes procedure for RNA, and is not valid for DNA. At least, the subsection title should be ammended. --AngelHerraez (talk) 11:58, 3 March 2011 (UTC)[reply]

I changed: "... and separates rRNA from ribosomes" to "... and separates rRNA from ribosomal proteins".

Furthermore: "... while phenol, isopropanol and water are solvents with poor solubility" --> poor solubility for what? This sentence is unclear.

--Felix Tritschler (talk) 17:17, 8 October 2012 (UTC)[reply]

Are you sure about the role of guanidine salts in phenol-based nucleic acid-extraction? This contradicts most protocols for phenol-extraction. Most of these protocols do not use guaninide salts at all. Phenol itself is already a chaotrophic agent. Also, according to most extraction protocols, the separation of RNA and DNA is dependent on the pH of the phenol solution (not any additional chaotrophic salts). At low pH mostly RNA is found in the aqueous phase, at high pH both RNA and DNA are found in the aqueous phase.134.76.70.252 (talk) 16:39, 4 February 2013 (UTC)[reply]

External links modified[edit]

Hello fellow Wikipedians,

I have just modified one external link on Acid guanidinium thiocyanate-phenol-chloroform extraction. Please take a moment to review my edit. If you have any questions, or need the bot to ignore the links, or the page altogether, please visit this simple FaQ for additional information. I made the following changes:

Added archive https://web.archive.org/web/20080509131137/http://www.mrcgene.com/tri.htm to http://www.mrcgene.com/tri.htm

When you have finished reviewing my changes, please set the checked parameter below to true or failed to let others know (documentation at {{Sourcecheck}}).

This message was posted before February 2018. After February 2018, "External links modified" talk page sections are no longer generated or monitored by InternetArchiveBot. No special action is required regarding these talk page notices, other than regular verification using the archive tool instructions below. Editors have permission to delete these "External links modified" talk page sections if they want to de-clutter talk pages, but see the RfC before doing mass systematic removals. This message is updated dynamically through the template {{source check}} (last update: 18 January 2022).

If you have discovered URLs which were erroneously considered dead by the bot, you can report them with this tool.
If you found an error with any archives or the URLs themselves, you can fix them with this tool.

Cheers.—InternetArchiveBot (Report bug) 12:04, 3 October 2016 (UTC)[reply]