Talk:Intein

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Untitled[edit]

Inteins....how is it now that they replicate???--66.191.21.251 04:42, 16 Feb 2005 (UTC)

I know very little about this field however, is it strictly correct to term them parasitic! Since both intein and extein can fulfill normal functions e.g the VME intein in S.Cerevisiae forms part of the vacuolar ATPase. Surely it just presents yet another way to get a protein product from a gene. This is not wholey unlike splicing, where exons can go to form proteins and sometimes introns can be used as snRNAs (i.e. functional products, not always junk) —Preceding unsigned comment added by 129.67.118.224 (talk) 10:35, 27 May 2008 (UTC)[reply]

Re: "Most reported inteins also contain an endonuclease domain that plays a role in intein propagation." How does this endonuclease activity play its role in propagation? How do inteins propagate? Briancady413 (talk) 15:49, 20 May 2009 (UTC)[reply]

re: "Full and mini inteins" How can an intein, a protein, guide the sequence of a DNA strand during action of the DNA repair mechanism induced by the endonuclease activity of the intein? Briancady413 (talk) 15:56, 20 May 2009 (UTC)[reply]

re:"Split inteins" How are the precursors to the split intein joined? Aren't they formed at distant-from-each-other locations? Briancady413 (talk) 16:01, 20 May 2009 (UTC)[reply]

Mistakes in the mechanism image!

There are two mistakes in the mechanism desribed. 1) In the caption, it should be saying "The C-extein is blue". 2) The black "X" residue in steps 3 & 4 should be protonated.

Best, OlgaMats (talk) 19:38, 8 January 2012 (UTC)[reply]

The mechanism also is missing arrows in several steps i.e. a shorthand double headed arrow could be added to the carbonyl oxygen to show substitution here (unless the aim was to simplify things) 129.215.5.253 (talk) 13:12, 1 May 2013 (UTC) DH[reply]