Trans-splicing

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Trans-splicing is a special form of RNA processing in eukaryotes where exons from two different primary RNA transcripts are joined end to end and ligated.

In contrast "normal" (cis-)splicing processes a single molecule. That is, trans-splicing results in an RNA transcript that came from multiple RNA polymerases on the genome. This phenomenon can be exploited for molecular therapy to address mutated gene products.[1]

Trans-splicing can be the mechanism behind certain oncogenic fusion transcripts.[2][3]

Trans-splicing is used by certain microbial organisms, notably protozoa of the Kinetoplastae class to produce variable surface antigens and change from one life stage to another.

References[edit]

  1. ^ Iwasaki, R; Kiuchi, H; Ihara, M; Mori, T; Kawakami, M; Ueda, H (2009). "Trans-splicing as a novel method to rapidly produce antibody fusion proteins". Biochemical and biophysical research communications 384 (3): 316–21. doi:10.1016/j.bbrc.2009.04.122. PMID 19409879. 
  2. ^ Li et al.; Wang, J.; Mor, G.; Sklar, J. (2008). "A neoplastic gene fusion mimics trans-splicing of RNAs in normal human cells". Science 321 (5894): 1357–61. doi:10.1126/science.1156725. PMID 18772439. 
  3. ^ Rickman et al.; Pflueger, D.; Moss, B.; Vandoren, V. E.; Chen, C. X.; De La Taille, A.; Kuefer, R.; Tewari, A. K. et al. (2009). "SLC45A3-ELK4 is a novel and frequent erythroblast transformation-specific fusion transcript in prostate cancer". Cancer Res. 69 (7): 2734–8. doi:10.1158/0008-5472.CAN-08-4926. PMID 19293179. 
  • Dixon RJ, Eperon IC, Samani NJ (2007). "Complementary intron sequence motifs associated with human exon repetition: a role for intragenic, inter-transcript interactions in gene expression". Bioinformatics 23 (2): 150–5. doi:10.1093/bioinformatics/btl575. PMID 17105720. 
  • Yang Y, Walsh CE (2005). "Spliceosome-mediated RNA trans-splicing". Mol. Ther. 12 (6): 1006–12. doi:10.1016/j.ymthe.2005.09.006. PMID 16226059. 
  • Coady TH, Shababi M, Tullis GE, Lorson CL (2007). "Restoration of SMN Function: Delivery of a Trans-splicing RNA Re-directs SMN2 Pre-mRNA Splicing". Molecular Therapy 15 (8): 1471–8. doi:10.1038/sj.mt.6300222. PMID 17551501. 
  • Wally V, Murauer EM, Bauer JW (2012). "Spliceosome-mediated trans-splicing: the therapeutic cut and paste.". J Invest Dermatol. 132 (8): 1959–66. doi:10.1038/jid.2012.101. PMID 22495179.