Trans-splicing
From Wikipedia, the free encyclopedia
Trans-splicing is a special form of RNA processing in eukaryotes where exons from two different primary RNA transcripts are joined end to end and ligated.
In contrast "normal" (cis-)splicing processes a single molecule. That is, trans-splicing results in an RNA transcript that came from multiple RNA polymerases on the genome. This phenomenon can be exploited for molecular therapy to address mutated gene products.
Trans-splicing can be the mechanism behind certain oncogenic fusion transcripts.[1][2]
Trans-splicing is used by certain microbial organisms, notably protozoa of the Kinetoplastae class to produce variable surface antigens and change from one life stage to another.
[edit] References
- ^ Li et al. (2008). A neoplastic gene fusion mimics trans-splicing of RNAs in normal human cells. doi:10.1126/science.1156725. PMID 18772439
- ^ Rickman et al. (2009). SLC45A3-ELK4 is a novel and frequent erythroblast transformation-specific fusion transcript in prostate cancer. doi:10.1158/0008-5472.CAN-08-4926. PMID 19293179
- Dixon RJ, Eperon IC, Samani NJ (2007). "Complementary intron sequence motifs associated with human exon repetition: a role for intragenic, inter-transcript interactions in gene expression". Bioinformatics 23 (2): 150–5. doi:10.1093/bioinformatics/btl575. PMID 17105720.
- Yang Y, Walsh CE (2005). "Spliceosome-mediated RNA trans-splicing". Mol. Ther. 12 (6): 1006–12. doi:10.1016/j.ymthe.2005.09.006. PMID 16226059.
- Coady TH, Shababi M, Tullis GE, Lorson CL (2007). "Restoration of SMN Function: Delivery of a Trans-splicing RNA Re-directs SMN2 Pre-mRNA Splicing". Molecular Therapy 15 (8): 1471–8. doi:10.1038/sj.mt.6300222. PMID 17551501.
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