User:JustinCHEM446/Outline
Protein Adsorption
Trevor Shepherd, Justin Hui, Jessica Cho
Methods to investigate protein adsorption[edit]
1. Fluorescent labeling / Radio labeling
2. Atomic Force Microscopy (AFM)
- Measures the force of adhesion between a protein-coated probe and the arrayed polymers.
3. Ceramics: 2D PAGE
4. Surface-Matrix Assisted Laser Desorption Ionization Mass Spectrometry (Surface-MALDIMS)[1]
- Instrument uses a pulsed UV laser for protein ionization and delayed extraction combined with time-of-flight analysis for mass separation
Metals[edit]
Relevance[edit]
1. Sterilization of implants and medical devices (reduced risk of disease transmission
- a. Surgical tool design
2. Reduced risk of corrosion
3. Engineer devices to have a lower risk of rejection after implantation
- a. Incorporating native proteins will reduce the host immune and inflammatory response
Chemical composition[edit]
1. Crystalline structure
- a. Crystal packing exposes potential metal-ion-dependent adhesion site (MIDAS) on one edge of the beta-sandwich and may serve as a binding site for collagens and other ligands. [2]
Coordination number[edit]
IMFs[edit]
Polymers[edit]
Chemical composition[edit]
Coordination number[edit]
IMFs[edit]
1. Dispersion
2. Dipole-dipole
3. Hydrogen Bonding
Ceramics[edit]
Chemical composition[edit]
Coordination number[edit]
IMFs[edit]
Effect of surface properties on adsorption[edit]
Surface wettability[edit]
1. Contact angle
2. Surface energy