Wisconsin Highway 65
|Maintained by WisDOT|
|Length:||54.12 mi (87.10 km)|
|South end:||US 10 / US 63 in Ellsworth|
| I-94 in Roberts
US 12 in Roberts
|North end:||US 8 in St. Croix Falls|
|Counties:||Pierce, St. Croix, Polk|
State Highway 65 begins at the corner of West Main Street (also known as U.S. Highway 10) and North Maple Street in downtown Ellsworth in Pierce County. WIS 65 heads north on Maple Street out of town, splitting off with County Highway J north of the Ellsworth Country Club golf course.
WIS 65 then heads in a generally west-northwesterly direction towards River Falls. At its intersection with WIS 29 at East Cascade Avenue, it is concurrently marked with WIS 35 north to its interchange with North Main Street on the northeast side of town. WIS 35 continues to the north while WIS 65 turns northeast on North Main and out of town.
WIS 65 runs parallel to the Kinnickinnic River along its northern banks until reaching North River Road outside of River Falls. WIS 65 then turns due north to its interchange with I-94 near Twin Lakes (Exit 10).
North of I-94, WIS 65 heads north as 120th Street into the village of Roberts where it intersects with U.S. Highway 12. WIS 65 then heads east with US 12 for about four blocks before heading north on 130th Street.
WIS 65 continues due north into the city of New Richmond, where it becomes South Knowles Avenue. The street is named for the former Wisconsin state governor, Warren P. Knowles, who lived in the city. WIS 65 continues through the city, becoming North Knowles Avenue after crossing the Willow River. WIS 65 intersects with WIS 64 to the northeast of the city, near Hatfield Lake and New Richmond Municipal Airport.
WIS 65 heads due north again to the village of Star Prairie, where it enters the village as Jewell Street. At its intersection with Hill Avenue (also known as County Highway H), WIS 65 curves to the west for a block before turning north onto Main Street. WIS 65 runs north for three blocks on Main Street before turning east again on Jerdee Avenue for a block, then continuing north on Jewell Street to the Polk/St. Croix County line.
WIS 65 turns to the northeast for several miles before turning again towards the north at its intersection with County Highway C in Polk County. WIS 65 continues north through a mostly rural part of the state, finally reaching its northern terminus with U.S. Highway 8 near Deer Lake, east of St. Croix Falls.
Bessert  notes that this last section of WIS 65, added in 1947, was not paved until 1950.