TCEP: Difference between revisions
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'''TCEP''' ('''''tris''(2-carboxyethyl)phosphine''') is a [[reducing agent]] frequently used in [[biochemistry]] and [[molecular biology]] applications. It is often prepared and used as a hydrochloride salt. |
'''TCEP''' ('''''tris''(2-carboxyethyl)phosphine''') is a [[reducing agent]] frequently used in [[biochemistry]] and [[molecular biology]] applications. It is often prepared and used as a hydrochloride salt. TCEP is also available as a stabilized solution at neutral pH and immobilized onto an agarose support to facilitate removal of the reducing agent. |
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==Applications== |
==Applications== |
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TCEP is often used as a reducing agent to break [[disulfide bond]]s within and between [[protein]]s as a preparatory step for [[gel electrophoresis]]. |
TCEP is often used as a reducing agent to break [[disulfide bond]]s within and between [[protein]]s as a preparatory step for [[gel electrophoresis]]. |
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Compared to the other two most common agents used for this purpose ([[dithiothreitol]] (DTT) and [[2-mercaptoethanol|β-mercaptoethanol]]) TCEP has the advantage of being odourless. It is also more resistant to oxidation in air than is DTT. |
Compared to the other two most common agents used for this purpose ([[dithiothreitol]] (DTT) and [[2-mercaptoethanol|β-mercaptoethanol]]), TCEP has the advantage of being odourless. It is also more more hydrophilic and more resistant to oxidation in air than is DTT. |
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==External links== |
==External links== |
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Revision as of 21:08, 28 June 2007
| tris(2-carboxyethyl)phosphine hydrochloride | |
|---|---|
| Chemical name | tris(2-carboxyethyl)phosphine hydrochloride |
| Other names | TCEP |
| Chemical formula | C9H16O6PCl |
| Molecular mass | 286.65 g mol−1 |
| CAS number | [51805-45-9] |
| Disclaimer and references | |
TCEP (tris(2-carboxyethyl)phosphine) is a reducing agent frequently used in biochemistry and molecular biology applications. It is often prepared and used as a hydrochloride salt. TCEP is also available as a stabilized solution at neutral pH and immobilized onto an agarose support to facilitate removal of the reducing agent.
Applications
TCEP is often used as a reducing agent to break disulfide bonds within and between proteins as a preparatory step for gel electrophoresis.
Compared to the other two most common agents used for this purpose (dithiothreitol (DTT) and β-mercaptoethanol), TCEP has the advantage of being odourless. It is also more more hydrophilic and more resistant to oxidation in air than is DTT.
External links
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