DNA laddering: Difference between revisions
Content deleted Content added
m Date/fix the maintenance tags or gen fixes |
David.Throop (talk | contribs) TUNEL assay |
||
| Line 8: | Line 8: | ||
* {{cite web|title= An evaluation of renal tubular DNA laddering in response to oxygen deprivation and oxidant injury|author=M Iwata, D Myerson, B Torok-Storb and RA Zager |url=http://jasn.asnjournals.org/cgi/content/abstract/5/6/1307|year=1996|accessdate=17 April|accessyear=2006}} |
* {{cite web|title= An evaluation of renal tubular DNA laddering in response to oxygen deprivation and oxidant injury|author=M Iwata, D Myerson, B Torok-Storb and RA Zager |url=http://jasn.asnjournals.org/cgi/content/abstract/5/6/1307|year=1996|accessdate=17 April|accessyear=2006}} |
||
==See Also== |
|||
*[[TUNEL assay]] |
|||
[[Category:Cell cycle]] |
[[Category:Cell cycle]] |
||
[[Category:Laboratory techniques]] |
[[Category:Laboratory techniques]] |
||
Revision as of 04:41, 3 February 2008
 | It has been suggested that this article be merged with DNA ladder. (Discuss) Proposed since October 2007. |
DNA laddering is a phenomenon seen in laboratory tests; it is a sensitive indicator of programmed cell death, specifically of apoptosis.
Endonuclease activation is a characteristic feature of apoptosis. This degrades genomic DNA at internucleosomal linker regions and produces 180- to 185- base-pair DNA fragments. On agarose gel electrophoresis, these give a characteristic "laddered" appearance. The dying cell's morphological changes are short-lived and difficult to detect. DNA laddering has therefore become a sensitive method to distinguish apoptosis from ischemic or toxic cell death.[1]
Citations
- M Iwata, D Myerson, B Torok-Storb and RA Zager (1996). "An evaluation of renal tubular DNA laddering in response to oxygen deprivation and oxidant injury". Retrieved 17 April.
{{cite web}}: Check date values in:|accessdate=(help); Unknown parameter|accessyear=ignored (|access-date=suggested) (help)CS1 maint: multiple names: authors list (link)
See Also
 | This genetics article is a stub. You can help Wikipedia by expanding it. |