Talk:G protein-gated ion channel

Peer review from BI481 Classmates

Comment 1

Hey guys,

I think your article is really well written and informative. The image you included is really helpful. I found your clinical significance section interesting, especially regarding breast cancer. I found another article indicating that mutations in G-protein-coupled receptor genes can cause them to be protooncogenes. The article looks pretty specific, but I don't think you would need to go into great detail, just mentioning the implication of tumorigenicity in your article would be interesting. The article is called

• Allen LF, Lefkowitz RJ, Caron MG, Cotecchia S (1991). "G-protein-coupled receptor genes as protooncogenes: constitutively activating mutation of the alpha 1B-adrenergic receptor enhances mitogenesis and tumorigenicity". Proc. Natl. Acad. Sci. U.S.A. 88 (24): 11354–8. PMC 53133. PMID 1662393. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)

Again, this is just a suggestion.

chengkd (talk) 8:38, 28 November 2009 (UTC)

Comment 2

This is a well put together article overall. You did an especially good job of identifying and distinguishing the different types of G protein-gated ion channels from one another and showing how their roles can differ greatly from one another. I think a beginner looking to gain knowledge on the subject would find your article to be useful for this explanation. Perhaps it could be made more useful with the addition of some external links to create a jumping-off point for further research. One point that could be added to make the article more complete would be how the functions of G protein-gated ion channels can vary under different conditions. One article that you may find useful concerning the acid sensitivity of a number of different types of ion channels (written by P. Holzer and published in the Handbook of experimental pharmacology) can be found here.

CharlesWCII^talk 19:15, 28 November 2009 (UTC)

Comment 3

I also enjoyed the article- it is very well written. I did have a few small suggestions: In "Function" section, it was a little confused by the first sentence "G protein-gated ion channels are a family of proteins involved in the direct activation of specific ion channels located in the plasma membrane of neurons." It gives the impression that ion channels activate ion channels which as the article makes clear, isn't the case. Also, I was unsure what the sentence "...which results in the departure of the βγ-subunit complex (Gβγ)" exactly. Additionally, it might also be helpful to include some specific examples of the muscular and neurological diseases caused by mutations.

Rabihgeha (talk) 20:27, 28 November 2009 (UTC)Rabihgeha

Comment 4

Hi guys! I read through your wiki article and found it very informative and well written. I thought for the most part it was easy to understand and the picture was a good addition- it helps to conceptualize everything you wrote in the article. Great job overall! One structural suggestion I would offer is in the section labeled "TYPE" it may be clearer if you had a consistent TYPE-STRUCTURE-FUNCTION-SUBTYPES motif; it may be harder to find information on some types of receptors but adding that consistency may make it easier to follow and understand the difference between the 4 types. Furthermore, after doing some outside research on the topic and reading some articles, I found information about G-protein coupled receptors being the target of many therapeutic drugs, I believe one article cited it as a target for up to 50% of marketed drugs. GPCR-signaling is another topic with which you could also expand your article. One such article titled "Towards a systems biology approach of G protein-coupled receptor signalling: Challenges and expectations" by Domitille Heitzler and others discusses how research of GPCR-signaling can lead to a discovery of new drug classes. Here is the link to the article:

• Heitzler D, Crépieux P, Poupon A, Clément F, Fages F, Reiter E (2009). "Towards a systems biology approach of G protein-coupled receptor signalling: challenges and expectations". C. R. Biol. 332 (11): 947–57. doi:10.1016/j.crvi.2009.09.002. PMID 19909918. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)

I hope you find my comments helpful!

Fdemsas (talk) 07:57, 29 November 2009 (UTC)

Comment 5

Great article - to echo what may have been touched upon by previous comments, I believe a section on current research could add to your article. You may also choose to integrate this within "Clinical Significance", possibly outlining how current research is trying improve treatment of neurological disorders. (still looking for articles, will provide resources once I find them) —Preceding unsigned comment added by Patrick.Raab.1 (talk • contribs) 21:22, 29 November 2009 (UTC)

Comment 6

Well written article. It is very clear and and the picture is definitely of assistance. One thing I thought you could touch up on was the section describing the relationship with chloride and sodium channel activity. I see that a lot is still not understood but I found an article that highlights a few more relationships G protein-gated ion channels may have with the individual channels. For instance, it give two possible mechanisms in which the G protein-gated ion channel inhibits the cardiac fast sodium currents. Just thought it might help. Otherwise, well done! Article can be found: http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6VS3-45478S5-26&_user=521319&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_searchStrId=1114353395&_rerunOrigin=scholar.google&_acct=C000026018&_version=1&_urlVersion=0&_userid=521319&md5=fbec46822ec209f73bcca8ba87f3f036

Nalvarez (talk) 17:49, 29 November 2009 (UTC)

Comment 7

This article is good thus far. However, I came across another reference you may want to incorporate into the article concerning the precise role of G proteins in regulating ion channels. I know you touched upon this in the "Function" section, but the article I found also discusses how G proteins mediate ion channel regulation by stimulating or inhibiting phosphorylation pathways, initiating intracellular cascades leading to the elevation of cytosolic calcium levels, or by producing various lipid-derived compounds. I think this information would be a beneficial addition to what you have already discussed on the page. Here is the link: http://physrev.physiology.org/cgi/content/abstract/75/4/865

Bartond (talk) 00:04, 30 November 2009 (UTC)

Comment 8

I thought your article is very well done I just had a few comments. I think the wording in the Function section is a little awkward and may just want to rework that paragraph. In addition I thought maybe there could be more expansion on why Leu and His are such important residues in the protein and also what residues are important for interacting with K+. I also felt that the section on Calcium channels could use some expansion and I felt a little confused in the explanation. Also may want some concluding paragraph to some up current studies and why it is important. EPalmquist (talk) 00:19, 30 November 2009 (UTC)

Comment 9

You guys put a good amount of effort and research on this topic. I think your organization of the different types of the G protein-gated ion channels was good, especially because there are so many types! However, the article has a repetition of "function" for calcium channels; you can probably throw that as a subsection in the main Function section to make the organization more cohesive. In the Functions, you should add the macro-function of G protein-gated ion channels of responding to metabotropic receptors. As we went over recently in class, metabotropic, specifically G protein-coupled receptors play a big role in synapses, and obviously G protein-gated ion channels are the second part of metabotropic synapse signal cascades. Your article can also use a "Future research" section after Clinical significance to tie in literature regarding research on the channels and their associated pathologies. Another cool clinical significance of G protein-coupled receptors is pain-killing. I found two articles relating GIRK's role in analgesia: http://www.ncbi.nlm.nih.gov/pubmed/19756153?itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum&ordinalpos=3 and http://www.ncbi.nlm.nih.gov/pubmed/19940980?itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum&ordinalpos=1. Cool beans, great job! (Wangtron (talk) 01:31, 30 November 2009 (UTC))