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[[File:Bisulfite conversion.svg|thumb|center|750px|alt=When treated with sodium bisulfite, cytosine is converted to uracil, while methylated cytosine is unaffected.|[[Sodium_bisulfite#Bisulfite_DNA_sequencing|Bisulfite conversion]], changing unmethylated [[cytosine]] to [[uracil]], while [[5-methylcytosine]] remains unaffected by the treatment.]] |
[[File:Bisulfite conversion.svg|thumb|center|750px|alt=When treated with sodium bisulfite, cytosine is converted to uracil, while methylated cytosine is unaffected.|[[Sodium_bisulfite#Bisulfite_DNA_sequencing|Bisulfite conversion]], changing unmethylated [[cytosine]] to [[uracil]], while [[5-methylcytosine]] remains unaffected by the treatment.]] |
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[[File:Cobra workflow.svg|thumb| |
[[File:Cobra workflow.svg|thumb|right|500px|alt=Bisulfite conversion selectively changes unmethylated cytosine to uracil, while unaffecting methylated cytosine. PCR then amplifies the sequences, after which restriction digestion of CpG islands fragments originally methylated sequences, while originally methylated sequences are unaffected.|The first few steps of COBRA, and the molecular changes caused by each step.]] |
Revision as of 03:08, 16 February 2011
![When treated with sodium bisulfite, cytosine is converted to uracil, while methylated cytosine is unaffected.](http://upload.wikimedia.org/wikipedia/commons/thumb/b/bc/Bisulfite_conversion.svg/750px-Bisulfite_conversion.svg.png)
![Bisulfite conversion selectively changes unmethylated cytosine to uracil, while unaffecting methylated cytosine. PCR then amplifies the sequences, after which restriction digestion of CpG islands fragments originally methylated sequences, while originally methylated sequences are unaffected.](http://upload.wikimedia.org/wikipedia/commons/thumb/f/fe/Cobra_workflow.svg/500px-Cobra_workflow.svg.png)