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[[File:Cobra workflow.svg|thumb|right|500px|alt=Bisulfite conversion selectively changes unmethylated cytosine to uracil, while unaffecting methylated cytosine. PCR then amplifies the sequences, after which restriction digestion of CpG islands fragments originally methylated sequences, while originally methylated sequences are unaffected.|The first few steps of COBRA, and the molecular changes caused by each step to methylated and unmethylated CpG sites]] |
[[File:Cobra workflow.svg|thumb|right|500px|alt=Bisulfite conversion selectively changes unmethylated cytosine to uracil, while unaffecting methylated cytosine. PCR then amplifies the sequences, after which restriction digestion of CpG islands fragments originally methylated sequences, while originally methylated sequences are unaffected.|The first few steps of COBRA, and the molecular changes caused by each step to methylated and unmethylated CpG sites]] |
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[[File:Cobra quantification.svg|thumb|right|500px|alt=The quantification step of [[Wikipedia:Combined Bisulfite Restriction Analysis|Combined Bisulfite Restriction Analysis]], taking place after [[Wikipedia:restriction digest|restriction digest]]ion. The digested [[Wikipedia:PCR|PCR]] fragments are separated and visualized by [[Wikipedia:gel electrophoresis|gel electrophoresis]], and the [[Wikipedia:DNA methylation|DNA methylation]] level of the input DNA can be determined by quantifying the comparing the amount of DNA in the digested and undigested bands.|The final quantification step of COBRA, where the DNA methylation levels of the original input sample can be deterrmined by comparing and quantifying the number of digested and undigested fragments.]] |
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Revision as of 03:16, 16 February 2011
