Acrydite: Difference between revisions
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Acrydite is a proprietary phosphoramidite that is capable of free-radical copolymerization with [[acrylamide]]. It can be used in a standard [[DNA synthesizer]] to add copolymerizable groups at the 5' terminus of any [[oligonucleotide]]. When preparing an [[polyacrylamide gel]], Acrydite capture probes are mixed in and polymerized into gel layers using standard methods. Acrydite-modified probes are also efficiently used in [[PCR]], and the resulting PCR products can be copolymerized into gels for use as capture probes. |
'''Acrydite''' is a proprietary phosphoramidite that is capable of free-radical copolymerization with [[acrylamide]]. It can be used in a standard [[DNA synthesizer]] to add copolymerizable groups at the 5' terminus of any [[oligonucleotide]]. When preparing an [[polyacrylamide gel]], Acrydite capture probes are mixed in and polymerized into gel layers using standard methods. Acrydite-modified probes are also efficiently used in [[PCR]], and the resulting PCR products can be copolymerized into gels for use as capture probes. |
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==Acrydite and DNA Computing== |
==Acrydite and DNA Computing== |
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Revision as of 20:48, 28 March 2006
Acrydite is a proprietary phosphoramidite that is capable of free-radical copolymerization with acrylamide. It can be used in a standard DNA synthesizer to add copolymerizable groups at the 5' terminus of any oligonucleotide. When preparing an polyacrylamide gel, Acrydite capture probes are mixed in and polymerized into gel layers using standard methods. Acrydite-modified probes are also efficiently used in PCR, and the resulting PCR products can be copolymerized into gels for use as capture probes.
Acrydite and DNA Computing
Recent experiments by Braich et al solving the Satisfiability Problem have demonstrated that Acrydite can be used in DNA computing to implement the extraction operation. Previously, a biotin-avidin magnetic bead system was used for the extraction operation.
References
- Mary Kenney, Satyajit Ray and T. Christian Boles (1998). "Mutation Typing Using Electrophoresis and Gel-Immobilized Acrydite(TM) Probes". Biotechniques (25): 516–521.
{{cite journal}}: Check date values in:|date=(help) — A paper about Acrydite and its applications. - Ravinderjit S. Braich; et al. (2000). "Solution of a Satisfiability Problem on a Gel-Based DNA Computer" (PDF). Lecture Notes In Computer Science. 2054: 27–42.
{{cite journal}}: Check date values in:|date=(help); Explicit use of et al. in:|author=(help) — A paper describing an experiment solving a 6-variable 3-SAT problem. - Ravinderjit S. Braich; et al. (2002). "Solution of a 20-Variable 3-SAT Problem on a DNA Computer". Science (journal). 296: 499–502.
{{cite journal}}: Check date values in:|date=(help); Explicit use of et al. in:|author=(help) — A paper describing an experiment solving a 20-variable 3-SAT problem.