Phosphate-buffered saline: Difference between revisions
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==Applications== |
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==Preparation== |
==Preparation== |
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The simplest way to prepare a PBS solution is to use PBS buffer tablets. They are formulated to give a ready to use PBS solution upon dissolution in a specified quantity of distilled water. They are available in the standard volumes: 100, 200, 500 and 1000 mL.<ref>[http://www.medicago.se/sites/default/files/pdf/productsheets/PBS_Buffer_v._01.pdf Medicago AB, (2010) Phosphate buffered saline specification sheet]</ref> |
The simplest way to prepare a PBS solution is to use PBS buffer tablets. They are formulated to give a ready to use PBS solution upon dissolution in a specified quantity of distilled water. They are available in the standard volumes: 100, 200, 500 and 1000 mL.<ref>[http://www.medicago.se/sites/default/files/pdf/productsheets/PBS_Buffer_v._01.pdf Medicago AB, (2010) Phosphate buffered saline specification sheet]</ref> |
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If used in cell culturing, the solution can be dispensed into aliquots and sterilized by autoclaving (20 min, 121°C, liquid cycle). Sterilization may not be necessary depending on its use. PBS can be stored at room temperature, but may warrant refrigeration to prevent bacterial growth if the solution is not sterile and is kept for long periods of time. However, concentrated stock solutions may precipitate when cooled and should be kept at room temperature until precipitate has completely dissolved before use. |
If used in cell culturing, the solution can be dispensed into aliquots and sterilized by [[autoclaving]] (20 min, 121°C, liquid cycle). Sterilization may not be necessary depending on its use. PBS can be stored at room temperature, but may warrant refrigeration to prevent bacterial growth if the solution is not sterile and is kept for long periods of time. However, concentrated stock solutions may [[precipitate]] when cooled and should be kept at room temperature until precipitate has completely dissolved before use. |
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==See also== |
==See also== |
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Revision as of 18:46, 16 April 2013
Phosphate buffered saline (PBS) is an osmoticly balanced buffer solution which has many uses biological laboratories. It can be used as a simple growth medium for microorganisms. It can also serve as a diluent in cell culture and other applications.
Preparation
There are many different ways to prepare PBS. Some formulations do not contain potassium, while others contain calcium or magnesium.[1] Generally, PBS contains the following constituents:
| Salt | Concentration | Concentration |
|---|---|---|
| (—) | (mmol/L) | (g/L) |
| NaCl | 137 | 8.01 |
| KCl | 2.7 | 0.20 |
| Na2HPO4 • 2 H2O | 10 | 1.78 |
| KH2PO4 | 2.0 | 0.27 |
| pH | 7.4 | 7.4 |
The simplest way to prepare a PBS solution is to use PBS buffer tablets. They are formulated to give a ready to use PBS solution upon dissolution in a specified quantity of distilled water. They are available in the standard volumes: 100, 200, 500 and 1000 mL.[2]
If used in cell culturing, the solution can be dispensed into aliquots and sterilized by autoclaving (20 min, 121°C, liquid cycle). Sterilization may not be necessary depending on its use. PBS can be stored at room temperature, but may warrant refrigeration to prevent bacterial growth if the solution is not sterile and is kept for long periods of time. However, concentrated stock solutions may precipitate when cooled and should be kept at room temperature until precipitate has completely dissolved before use.
See also
References
- ^ Dulbecco, R. et al. (1954): Plaque formation and isolation of pure lines with poliomyelitis viruses. In: J. Exp. Med. vol. 99 (2), pp. 167-182. doi:10.1084/jem.99.2.167 PMID 13130792
- ^ Medicago AB, (2010) Phosphate buffered saline specification sheet