SYBR Green I
SYBR Green I (SG) is an asymmetrical cyanine dye used as a nucleic acid stain in molecular biology. SYBR Green I binds to double-stranded DNA. The resulting DNA-dye-complex absorbs blue light (λmax = 498 nm) and emits green light (λmax = 522 nm). SYBR Green I could replace the very strong mutagen ethidium bromide.
Similar cyanine dyes:
- SYBR Green II
- SYBR Gold
- YO (Oxazole Yellow)
- TO (Thiazole Orange)
- PG (PicoGreen)
Literature
Zipper, H. et al. (2004). Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications. Nucleic Acids Res. 32, e103. PMID 15249599 PDF