User:HermiaStudy/Pseudomonas aeruginosa

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Article Draft

Lead

Changes

The article already had an identification table, so I moved the information to other format. I added the required missing test results from my reference that the table didn't already cover. Because I had originally been doing my work on the Pseudomonas genus article (https://en.wikipedia.org/wiki/User:HermiaStudy/Pseudomonas?veaction=edit&preload=Template%3ADashboard.wikiedu.org_draft_template), I had originally done some editing but not too much. I wrote my paragraph for this article under the classification tab and described some differences in the colony morphology in the species (my addition to the paragraph is in brackets and was published 4/21/22).

Identification (OLD TABLE)

Test	Results
Gram Stain	-
Oxidase	+
Indole Production	-
Methyl Red	-
Voges-Proskauer	-
Citrate	+
Hydrogen Sulfide Production	-
Urea Hydrolysis	-
Phenylalanine Deaminase	-
Lysine Decarboxylase	-
Motility	+
Gelatin Hydrolysis	+
acid from lactose	-
acid from glucose	+
acid from maltose	-
acid from mannitol	+
acid from sucrose	-
nitrate reduction	+
DNAse	-
Lipase	+
Pigment (X)	+ (bluish green pigmentation)
Catalase	+
Hemolysis (X)	Beta/variable

Classification

Morphological, physiological, and biochemical characteristics of Pseudomonas aeruginosa are shown in the Table below.

Test type	Test	Characteristics
Colony characters	Size	Large
	Type	Smooth
	Color
	Shape	Flat
Morphological characters	Shape	Rod
Physiological characters	Motility	+
Physiological characters	Growth at 6.5% NaCl	-
Biochemical characters	Gram staining	-
	Oxidase	+
	Catalase	+
	Oxidative-Fermentative
	Motility	+
	Methyl Red	-
	Voges-Proskauer	-
	Indole	-
	H₂S Production	-
	Urease	-
	Nitrate reductase	+
	β-Galactosidase
	Phenylalanine Deaminase	-
	DNAse	-
	Lipase	+
	Lysine Decarboxylase	-
	Pigment	+ (bluish green pigmentation)
	Hemolysis	Beta/variable
Hydrolysis of	Gelatin	+

	Casein



Utilization of	Glycerol	+
	Galactose	-
	D-Glucose	+
	D-Fructose	+
	D-Mannose	-
	Mannitol	+
	Citrate	+
	Maltose	-
	Sucrose	-
	Lactose	-

Note: + = Positive, – =Negative

Identification of P. aeruginosa can be complicated by the fact individual isolates often lack motility. [The colony morphology itself also displays several varieties. The main two types are large, smooth, with a flat edge and elevated center and small, rough, and convex.^[1] A third type, mucoid, can also be found. The large colony can typically be found in clinal settings while the small is found in nature.^[1] The third, however, is present in biological settings and has been found in respiratory and in the urinary tract.^[1]] Furthermore, mutations in the gene lasR drastically alter colony morphology and typically lead to failure to hydrolyze gelatin or hemolyze.^{[citation needed]}

References

Murray, R.G.E.; Holt, John G. (2005), "The History of Bergey's Manual", Bergey’s Manual® of Systematic Bacteriology, Boston, MA: Springer US, pp. 1–14, ISBN 978-0-387-24143-2, retrieved 2022-04-21

^ ^a ^b ^c Murray, R.G.E.; Holt, John G. (2005), "The History of Bergey's Manual", Bergey’s Manual® of Systematic Bacteriology, Boston, MA: Springer US, pp. 1–14, ISBN 978-0-387-24143-2, retrieved 2022-04-21

[:0-1] Murray, R.G.E.; Holt, John G. (2005), "The History of Bergey's Manual", Bergey’s Manual® of Systematic Bacteriology, Boston, MA: Springer US, pp. 1–14, ISBN 978-0-387-24143-2, retrieved 2022-04-21

[1]