Jump to content

TEV protease

From Wikipedia, the free encyclopedia

This is an old revision of this page, as edited by 128.104.112.117 (talk) at 21:52, 5 May 2009 (edit wording, link cysteine protease, TEV). The present address (URL) is a permanent link to this revision, which may differ significantly from the current revision.

The TEV protease is a highly site-specific cysteine protease that is found in the Tobacco Etch Virus (TEV). The optimum recognition site for this enzyme is the sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly and cleavage occurs between the Gln and Gly residues. Some of the advantages of this enzyme are its high specificity and its high activity rate. One of the main uses of this protein is for removing affinity tags from purified proteins.

M.W. = 25 to 27 kDa , depends on the specific construct used.

References

TEV Protease Homepage

Invitrogen's TEV Protease Product Page