Ramachandran plot
A Ramachandran plot (also known as a Ramachandran map or a Ramachandran diagram), developed by Gopalasamudram Narayana Ramachandran, is a way to visualize dihedral angles φ against ψ of amino acid residues in protein structure. It shows the possible conformations of φ and ψ angles for a polypeptide.
Mathematically, the Ramachandran plot is the visualization of a function . The domain of this function is the torus. Hence, the conventional Ramachandran plot is a projection of the torus on the plane, resulting in a distorted view and the presence of discontinuities.
One would expect that larger side chains would result in more restrictions and consequently a smaller allowable region in the Ramachandran plot. In practice this does not appear to be the case; only the methylene group at the β position has an influence. Glycine has a hydrogen atom, with a smaller van der Waals radius, instead of a methyl group at the β position. Hence it is least restricted and this is apparent in the Ramachandran plot for Glycine for which the allowable area is considerably larger.
In contrast, the Ramachandran plot for proline shows only a very limited number of possible combinations of ψ and φ.
The Ramachandran plot was calculated just before the first protein structures at atomic resolution were determined. Forty years later there were tens of thousands of high-resolution protein structures determined by X-ray crystallography and deposited in the Protein Data Bank (PDB). From one thousand different protein chains, Ramachandran plots of over 200 000 amino acids were plotted, showing some significant differences, especially for glycine (Hovmöller et al. 2002). The upper left region was found to be split into two; one to the left containing amino acids in beta sheets and one to the right containing the amino acids in random coil of this conformation.
One can also plot the dihedral angles in polysaccharides and other polymers in this fashion. For the first two protein side-chain dihedral angles a similar plot is the Janin Plot.
Software
- Web-based tool showing Ramachandran plot of any PDB entry
- STING
- Pymol with the DynoPlot extension
- VMD, distributed with dynamic Ramachandran plot plugin
- Sirius
- Swiss PDB Viewer
- TALOS
See also PDB for a list of similar software.
References
- G.N. Ramachandran, C. Ramakrishnan & V. Sasisekharan (1963): Stereochemistry of polypeptide chain configurations. In: J. Mol. Biol. vol. 7, p. 95-99. PMID 13990617
- S. Hovmöller, T. Zhou & T. Ohlson (2002) Conformations of amino acids in proteins. In: Acta Cryst. vol. D58, p. 768-776.