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User:Jujubix

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This is an old revision of this page, as edited by Jujubix (talk | contribs) at 03:16, 16 February 2011. The present address (URL) is a permanent link to this revision, which may differ significantly from the current revision.

When treated with sodium bisulfite, cytosine is converted to uracil, while methylated cytosine is unaffected.
Bisulfite conversion, changing unmethylated cytosine to uracil, while 5-methylcytosine remains unaffected by the treatment.
Bisulfite conversion selectively changes unmethylated cytosine to uracil, while unaffecting methylated cytosine. PCR then amplifies the sequences, after which restriction digestion of CpG islands fragments originally methylated sequences, while originally methylated sequences are unaffected.
The first few steps of COBRA, and the molecular changes caused by each step to methylated and unmethylated CpG sites
The quantification step of Combined Bisulfite Restriction Analysis, taking place after restriction digestion. The digested PCR fragments are separated and visualized by gel electrophoresis, and the DNA methylation level of the input DNA can be determined by quantifying the comparing the amount of DNA in the digested and undigested bands.
The final quantification step of COBRA, where the DNA methylation levels of the original input sample can be deterrmined by comparing and quantifying the number of digested and undigested fragments.
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