PtK2 cells

PtK₂ Cells are a cell line derived from male rat-kangaroo (Potorous tridactylis) epithelial kidney cells^[1]. This cell line is used for a variety of application in biomedical research but is particularly popular as a model for mitosis^[2] .

Origin

The PtK₂ cell line was established by Kristen Walen and Spencer Brown in 1962^[3]. Cells from the rat kangaroo were selected as the source of a cell line because this species has only a small number of chromosomes and these chromosomes are easily visualized under a microscope. This makes these cells particularly suited to studying mitosis.

Characteristics

PtK₂ cells are relatively large and when grown in a monolayer stay flat through out the cell cycle unlike many cells that round up during mitosis^[4] . PtK2 cells are resistant to adenovirus 5, coxsackievirus B5, and poliovirus 2. They are susceptible to coxsackievirus A9, herpes simplex, vaccinia, and vesicular stomatitis (Ogden strain)^[5] . PtK₂ cells contain intermediate filaments composed of Keratin^[6] .

References

^ "PtK2 (NBL-5) (ATCC® CCL-56™) Potorous tridactylis kidney norm". Retrieved 17 December 2013.
^ Elgin, edited by Barbara A. Hamkalo, Sarah C.R. (1991). Functional organization of the nucleus a laboratory guide. San Diego: Academic Press. p. 143. ISBN 008085933X. {{cite book}}: |first= has generic name (help)CS1 maint: multiple names: authors list (link)
^ WALEN, KH (1962 Apr 28). "Chromosomes in a marsupial (Potorous tridactylis) tissue culture". Nature. 194: 406. PMID 14004541. {{cite journal}}: Check date values in: |date= (help); Unknown parameter |coauthors= ignored (|author= suggested) (help)
^ "Molecular Expressions Microscopy Primer: Specialized Microscopy Techniques - Fluorescence Digital Image Gallery - Male Rat Kangaroo Kidney Epithelial Cells (PtK2)". Retrieved 17 December 2013.
^ Rosemarie, Lindl, Toni;Steubing,. Atlas of Living Cell Cultures. Weinheim: Wiley. p. 465. ISBN 3527669930.{{cite book}}: CS1 maint: extra punctuation (link) CS1 maint: multiple names: authors list (link)
^ "Olympus Microscopy Resource Center | Fluorescence Digital Image Gallery - Epithelial Cells". Retrieved 17 December 2013.

[1] "PtK2 (NBL-5) (ATCC® CCL-56™) Potorous tridactylis kidney norm". Retrieved 17 December 2013.

[2] Elgin, edited by Barbara A. Hamkalo, Sarah C.R. (1991). Functional organization of the nucleus a laboratory guide. San Diego: Academic Press. p. 143. ISBN 008085933X. {{cite book}}: |first= has generic name (help)CS1 maint: multiple names: authors list (link)

[3] WALEN, KH (1962 Apr 28). "Chromosomes in a marsupial (Potorous tridactylis) tissue culture". Nature. 194: 406. PMID 14004541. {{cite journal}}: Check date values in: |date= (help); Unknown parameter |coauthors= ignored (|author= suggested) (help)

[4] "Molecular Expressions Microscopy Primer: Specialized Microscopy Techniques - Fluorescence Digital Image Gallery - Male Rat Kangaroo Kidney Epithelial Cells (PtK2)". Retrieved 17 December 2013.

[5] Rosemarie, Lindl, Toni;Steubing,. Atlas of Living Cell Cultures. Weinheim: Wiley. p. 465. ISBN 3527669930.{{cite book}}: CS1 maint: extra punctuation (link) CS1 maint: multiple names: authors list (link)

[6] "Olympus Microscopy Resource Center | Fluorescence Digital Image Gallery - Epithelial Cells". Retrieved 17 December 2013.

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