User talk:Rcalleja/sandbox
Anti mRNA oligonucleotides Peer Review 1
First paragraph should be the “introduction” instead of the summary. AMOs are design to interfere with miRNA and so a direct link to “miRNA” on wiki is needed. AMOs can hybridize to miRNA and sterically block interactions. Both of these mechanisms should be mentioned in the introduction. The current introduction only mentions that AMOs steric block miRNA. This paper talks about AMOs in more detail AMOs-http://www.nature.com/mtna/journal/v2/n8/full/mtna201346a.html. The authors should also mention in the introduction that AMOs are used as therapeutics. Over expression, under expression or aberrations in miRNA can be lead to diseases. However, whether AMOs can be used to treat diseases due to any of theses conditions is not clearly mentioned. If AMOs can be used to any of these, it should be clearly explained. Another unaddressed question is whether AMOs can hybridize to other noncoding RNAs.
Its good to mention about both first generation and second generation AMOs in general. The paragraph under “disease” should be added to the introduction directly. Whether AMO is a novel treatment is not clear. Are there any drawbacks of using AMOs as therapeutics?
"By minimizing overexpression with AMOs and fixing underexpression with reinsertions of missing genes, some of these genetic diseases can be fixed or at least minimize their symptom” is a different application of AMOs. It seems to integrate with genes rather than hybridize to miRNA. Therefore, this should be mentioned as another application of AMOs.
"Many human diseases have been found to have some expression involving miRNA”. Here, it should be corrected as aberrations or alterations in expression levels of miRNA leads to many diseases such as….etc. "By targeting cells affected with improper miRNA expression, the normal balance of the expression can be restored by using AMOs”. This sentence seems pretty confusing and could be omitted. Disease section is good to be changed as therapeutics. Also under therapeutics, the author needs to talk more about miRNA and then mention how AMOs are used to…”. For an example in Neurogeneration, its good to start with something like "AMOs have been applied to study miRNA level and activity in neurogenesis" and then proceed to further explain their application.
How AMOs are delivered into cells needs to be mentioned. Also how successful treatments are, should be addressed.
Scristie (talk) 22:40, 22 October 2014 (UTC)
Suggestions from ChemLibrarian
Good work! A few more suggestions here.
1. I fixed your link on the course page to the original article page. Just want to let you know that the link you posted didn't work because of different capitalization of the word. It should be [[RNA helicase | RNA Helicase]] if you'd like to keep the H capitalized in your text.
2. When you are ready to move your article to the real article page, you don't need the "Summary" section heading. That paragraph is just the lead section.
3. As you see that the figure you used doesn't show up any more. Looks like your syntax looks right. But just could not find the images you refer to on the Wikimedia Commons site. Please make sure you have the right to post the image. An image directly form a publication is protected by copyright of the publisher and it's unlikely you can upload it here. I followed the link appeared there now and it seems like to be the case for your image. It muse have been deleted due to copyright issue. Please let me know if you need additional help. Besides the picture tutorial page mentioned by the other reviewer, you can also watch the video tutorial below.
4. If you don't have anything under the Detection section, please just delete the section.
5. You did not have many internal links to other Wikipedia articles for terms and jargon in your addition. e.g. cancers, muscular diseases ... Please add some internal links as you can.
Hope it helps. Please let me know if you have any questions. ChemLibrarian (talk) 18:44, 27 October 2014 (UTC)
GSI Comments
Hi Rcalleja,
Thank you for your work on this page. Please consider the points made by you reviewers when finishing your edits. Great job!
Elizabeth