Mustard Database

Mustard
Content
Description	Contains AR determinants and curated AR genes identified from metagenomics
Data types; captured	Antimicrobial resistance genes and AR Determinants
Organisms	Bacteria
Contact
Primary citation	PMID 30478291
Access
Website	mgps.eu/Mustard/
Download URL	Download
Miscellaneous
Bookmarkable; entities	yes

Mustard is a database that tracks Antimicrobial Resistance Determinants (ARDs). The method by which it tracks ARDs is using their own method adapted from Protein Homology Modelling called Pairwise Comparative Modelling (PCM), which increase specificity protein prediction, especially for distantly related protein homologues^[1]. Using PCM, 6095 ARDs from 20 families in the human gut microbiota. Antibiotic resistance databases used were ResFinder^[2], ARG-ANNOT^[3], the now defunct Lahey Clinic, RED-DB, Marilyn Roberts website for tetracycline and macrolide resistance genes and metagenomics^[4]^[5]^[6].

References:

^ Ehrlich, S. Dusko; Kennedy, Sean P.; Doré, Joël; Haimet, Florence; Weiszer, Kevin; Levenez, Florence; Letur, Aline; Léjard, Véronique; Léonard, Pierre (January 2019). "Prediction of the intestinal resistome by a three-dimensional structure-based method". Nature Microbiology. 4 (1): 112–123. doi:10.1038/s41564-018-0292-6. ISSN 2058-5276.
^ Zankari, E.; Hasman, H.; Cosentino, S.; Vestergaard, M.; Rasmussen, S.; Lund, O.; Aarestrup, F. M.; Larsen, M. V. (2012-07-10). "Identification of acquired antimicrobial resistance genes". Journal of Antimicrobial Chemotherapy. 67 (11): 2640–2644. doi:10.1093/jac/dks261. ISSN 0305-7453.
^ Gupta, Sushim Kumar; Padmanabhan, Babu Roshan; Diene, Seydina M.; Lopez-Rojas, Rafael; Kempf, Marie; Landraud, Luce; Rolain, Jean-Marc (2013-10-21). "ARG-ANNOT, a New Bioinformatic Tool To Discover Antibiotic Resistance Genes in Bacterial Genomes". Antimicrobial Agents and Chemotherapy. 58 (1): 212–220. doi:10.1128/aac.01310-13. ISSN 0066-4804.
^ Allen, Heather K; Moe, Luke A; Rodbumrer, Jitsupang; Gaarder, Andra; Handelsman, Jo (2008-10-09). "Functional metagenomics reveals diverse β-lactamases in a remote Alaskan soil". The ISME Journal. 3 (2): 243–251. doi:10.1038/ismej.2008.86. ISSN 1751-7362.
^ Moore, Aimée M.; Patel, Sanket; Forsberg, Kevin J.; Wang, Bin; Bentley, Gayle; Razia, Yasmin; Qin, Xuan; Tarr, Phillip I.; Dantas, Gautam (2013-11-13). "Pediatric Fecal Microbiota Harbor Diverse and Novel Antibiotic Resistance Genes". PLoS ONE. 8 (11): e78822. doi:10.1371/journal.pone.0078822. ISSN 1932-6203.{{cite journal}}: CS1 maint: unflagged free DOI (link)
^ Sommer, M. O. A.; Dantas, G.; Church, G. M. (2009-08-27). "Functional Characterization of the Antibiotic Resistance Reservoir in the Human Microflora". Science. 325 (5944): 1128–1131. doi:10.1126/science.1176950. ISSN 0036-8075.