List of DNA sequencers

The market of DNA sequencer instruments is currently dominated by: Illumina (December 2019), followed by PacBio, MGI/BGI and Oxford Nanopore. There are also offerings from Qiagen GeneReader (as of December 2019) and GenapSys. The majority of sequencers can also produce RNA sequences either directly or via cDNA preparation.

The main considerations for DNA sequencer instruments or sequencing runs are:

- Throughput.

- Cost per base.

- Quality of the basecalls.

- Read lengths / sequence lengths.

- Rate of production of data.

- Cost of the instrument.

Current platforms

An up-to-date table of current instruments still actively offered is shown:

Platform	Reads x run: (M)	Read length: (paired-end*, Half of data in reads**)	Run time: (d)	Yield: (Gb)	Rate: (Gb/d)	Reagents: ($)	per-Gb: ($)	hg-30x: ($)	Machine: ($)	Install base	Max theoretical output per day (Gb)	Max total output per day (Gb) from current installbase
iSeq 100 1fcell	4	150*	077-1.28	1.2	0.79	650	542	--	19.9K	350	1.56	546
MiniSeq 1fcell	25	150*	1	7.5	7.5	1750	218	28000	49.5K	900	7.5	6750
MiSeq 1fcell	25	300*	2	15	7.5	1000	108	8000	99K	6700	7.5	50250
NextSeq 550 1fcell	400	150*	1.2	120	100	5000	40	5000	250K	3000	100	300,000
HiSeq 2500 RR 2fcells	600	100*	1.125	120	106.6	6145	51.2	6144	740K	0	106.6	0
HiSeq 2500 V3 2fcells	3000	100*	11	600	55	23470	39.1	4692	690K	0	55	0
HiSeq 2500 V4 2fcells	4000	125*	2.5	1000	166	29900	37	3804	690K	1300	166	215,800
HiSeq 4000 2fcells	5000	150*	3.5	1500	400	--	24	2460	900K	400	400	160,000
HiSeq X 2fcells	6000	150*	3	1800	600	--	8	849.6	1M-1.2M	400	600	240,000
NovaSeq SP 2fcells	1600	250*	1.58	800	505.3	11500	14.4	2000	985K	0	600	0
NovaSeq S1 2fcells	3200	150*	1.66	1000	600	18750	13	1800	985K	0	600	0
NovaSeq S2 2fcells	6600	150*	1.66	2000	1200	35000	12	1564	985K	0	0	0
NovaSeq S4 2fcells*	20000	150*	1.83	6000	3600	64000	7.00	700	985K	600	3600	2,160,000
PacBio RSII	0.88	20K**	4.3	12	2.8	2400	200	24000	695K	100	2.8	280
PacBio 8M Sequel II v2.0 chem 1 flowcell	4	45K-185K**	1	30-180	--	1300	7.2-43.3	648-3897	350K	75	180	13,500
PacBio 48M chip R&D 1 flowcell	24	45K-185K**	1	180-1080	--	1300	1.2-7.2	108-648	350K	0	1080	0
SOLID 5500 XL	1400	60	7	180	30	10500	58.33	7000	595K	0	0	0
Ion S5 510 1chip	2 - 3	200-400	0.21	1	4.8	950	950	114000	65K	0	0	0
Ion S5 520 1chip	3 - 6	200-600	0.23	1	4.3	1000	500	60000	65K	0	0	0
Ion S5 530 1chip	20	200-600	0.29	4	13.8	1200	150	18000	65K	0	0	0
Ion S5 540 1chip	80	200	0.42	15	35.7	1400	93.3	11196	65k	0	0	0
Ion S5 550 1chip	130	200	0.5	25	50	1670	66.8	8016	65k	0	0	0
Plate Flongle 1fcell=1well (2020)	--	500-2000000	3	0.5/1	--	25/50	100-25	--	--	0	0	0
SmidgION 1fcell	--	500-2000000	TBC	TBC	TBC	TBC	TBC	--	--	0	0	0
Flongle 1fcell	--	500-2000000	1	0.1/1.8-3.3	--	--	90-30	8100-2700	--	0	0	0
MinION Mk 1B 1fcell	--	500-2000000	3	17/30-50	--	900-475	53-9.5	4770-885	--	4500	15	67,500
GridION X5 5fcells	--	500-2000000	3	85/150-250	--	900-475	53-9.5	4770-885	50K	100	75	7,500
PromethION 48fcells	--	500-2000000	2.6	3000/7000-15000	--	2000-625	22-6.95	1400-315	285K	45	5769	259,615
QiaGen GeneReader	48	100-150	--	7.2	--	500	--	--	--	0	0	0
DNBSEQ T7 4fcells	20000	150*	1	6000	6000	NA	5	500	--	13	6000	78,000
MGISEQ 2000	3600	150*	3	1440	480	7200	5	600	320K	135	480	64,800
BGISEQ 500	2600	100*	7	520	37	--	--	600?	240K	950	37	35,150
MGISEQ 200	300	100*	2	60	30	--	--	--	160K	25	30	750
Genapsys 1M Chip	1	150	1.17	0.15	0.1	100	667	80000	10K	1	30	30
Genapsys 16M Chip	10 - 12	150	1.17	1.65	NA	300	182	21818	10K	1	30	30
Genapsys 144M Chip	144	150	1.17	21.6	NA	600	28	3333	10K	1	30	30

Technologies under development

There are several new start-up and larger companies developing methods for DNA sequencing:

Company	Total Money Raised (MUSD)	Money Raised at IPO or Acquisition (MUSD)	Valuation in last raise or Market Cap (MUSD)	Company Valuation for IPOed companies (MUSD)	Further Info
Universal Sequencing Technology	8	0	0	0	http://41j.com/blog/2018/07/direct-genomics/
Quantum-SI	0	0	0	0	info
XGenomes	0	0	1	0	http://41j.com/blog/2018/12/quantumsi/
QuantuMDx	26.3	0	2	0	https://techcrunch.com/2019/03/15/xgenomes-is-bringing-dna-sequencing-to-the-masses/
iNanoBio	3.3	0	20	0	http://41j.com/blog/2018/09/quantumdx/
Centrillion Biosciences	24.3	0	54	0	http://41j.com/blog/2018/09/inanobio/
Direct Genomics	34.8	0	0	0	http://41j.com/blog/2018/07/centrillion-technologies/
Quantapore	50.6	0	93	0	info
Ontera	32.6		100	0	--
LaserGen (acquired)	85	105	105	0	--
Stratos Genomics	55.9	0	120	0	http://41j.com/blog/2018/11/stratos-genomics/
ReadCoor	25.5	0	138	0	--
Roswell Biotechnologies	38		192	0	http://41j.com/blog/2018/09/roswell-biotechnologies/
Omniome	60	0	360	0	info