2'-O-methylation

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2'-O-methyl-adenosine, a modified adenosine.

2'-O-methylation is a common nucleoside modification of RNA, where a methyl group is added to the 2' hydroxyl of the ribose moiety of a nucleoside, producing a methoxy group. 2'-O-methylated nucleosides are mostly found in ribosomal RNA and small nuclear RNA and occur in the functionally essential regions of the ribosome and splicesome.[1] Currently, about 1210 2'-O-methylations (2'-O-Me) have been identified in mammals and yeast and deposited in RMBase (RNA Modification Base) database.[2]

Having the chemical properties intermediate between RNA and DNA, 2'-O-methylation is presumed to have one of the reactive group of RNA molecules on early earth that would have given rise to DNA 1.

Recently a novel method to map 2'-O ribose methylations by high throughput sequencing has been published.[3] The method is quantitative and maps all modifications in a single experiment.

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References[edit]

  1. ^ Kiss T (2001). "Small nucleolar RNA-guided post-transcriptional modification of cellular RNAs". EMBO J. 20 (14): 3617–3622. doi:10.1093/emboj/20.14.3617. PMC 125535Freely accessible. PMID 11447102. 
  2. ^ Sun, WJ; Li, JH; Liu, S; Wu, J; Zhou, H; Qu, LH; Yang, JH (4 January 2016). "RMBase: a resource for decoding the landscape of RNA modifications from high-throughput sequencing data.". Nucleic acids research. 44 (D1): D259–65. PMID 26464443. 
  3. ^ Birkedal U et. al. (2015). "Profiling of Ribose Methylations in RNA by High-Throughput Sequencing". Angew. Chem. Int. Ed. 54 (2): 451–455. doi:10.1002/anie.201408362. PMID 25417815.