HMB-45

HMB-45 is a monoclonal antibody that reacts against an antigen present in melanocytic tumors such as melanomas, and stands for Human Melanoma Black. It is used in anatomic pathology as a marker for such tumors. The specific antigen recognized by HMB-45 is now known as Pmel 17.

History

HMB-45 was discovered by Drs. Allen M. Gown and Arthur M. Vogel in 1986.^[1] The antibody was generated to an extract of melanoma.

Cancer diagnostics

In a study to determine diagnostic usefulness of specific antibodies used to identify melanoma, HMB-45 had a 92% sensitivity when used to identify melanoma. The antibody also reacts positively against junctional nevus cells and fetal melanocytes.^[2]

Despite this relatively high sensitivity—HMB-45 does have its drawbacks. HMB-45 can be detected in only 50-70% of melanomas. HMB-45 does not react well against intradermal nevi, normal adult melanocytes, spindle cell melanomas and desmoplastic melanomas. HMB-45 is nonreactive with almost all non-melanoma human malignancies, with the exception of rare tumors showing evidence of melanogenesis (e.g., pigmented schwannoma, clear cell sarcoma) or tumors associated with tuberous sclerosis complex (angiomyolipoma and lymphangiomyoma).^[3]

Storage

HMB-45 should be stored at 4 degree Celsius. At 4 degrees Celsius the antibody will be stable for up to 2 months without any loss of quality. For best results it should be used before the expiration date.

Alternatives

When conducting an immunocytochemical studies to identify melanoma for scientific or clinical studies, scientist and medical professionals can also use S-100, Melan-A, Tyrosinase, and Mitf to identify tumors.

References

^ Gown AM, Vogel AM, Hoak D, Gough F, McNutt MA (1986). "Monoclonal antibodies specific for melanocytic tumors distinguish subpopulations of melanocytes". Am. J. Pathol. 123 (2): 195–203. PMC 1888307. PMID 3518473.
^ Ramgolam, K; Lauriol, J; Lalou, C; Lauden, L; Michel, L; de la Grange, P; Khatib, AM; Aoudjit, F; Charron, D; Alcaide-Loridan, C; Al-Daccak, R (2011). "Melanoma spheroids grown under neural crest cell conditions are highly plastic migratory/invasive tumor cells endowed with immunomodulator function". PLOS ONE. 6 (4): e18784. doi:10.1371/journal.pone.0018784. PMC 3078142. PMID 21526207.
^ Mahmood, M. N.; Lee, M. W.; Linden, M. D.; Nathanson, S. D.; Hornyak, T. J.; Zarbo, R. J. (2002). "Diagnostic Value of HMB-45 and Anti-Melan a Staining of Sentinel Lymph Nodes with Isolated Positive Cells". Modern Pathology. 15 (12): 1288–1293. doi:10.1097/01.MP.0000037313.33138.DF. PMID 12481009.

Sheffield, MV; Yee, H; Dorvault, CC; Weilbaecher, KN; Eltoum, IA; Siegal, GP; Fisher, DE; Chhieng, DC (2002). "Comparison of five antibodies as markers in the diagnosis of melanoma in cytologic preparations". Am. J. Clin. Pathol. 118 (6): 930–6. doi:10.1309/EWK9-LUPR-6BC5-1GXV. PMID 12472287.

External links

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[1] Gown AM, Vogel AM, Hoak D, Gough F, McNutt MA (1986). "Monoclonal antibodies specific for melanocytic tumors distinguish subpopulations of melanocytes". Am. J. Pathol. 123 (2): 195–203. PMC 1888307. PMID 3518473.

[2] Ramgolam, K; Lauriol, J; Lalou, C; Lauden, L; Michel, L; de la Grange, P; Khatib, AM; Aoudjit, F; Charron, D; Alcaide-Loridan, C; Al-Daccak, R (2011). "Melanoma spheroids grown under neural crest cell conditions are highly plastic migratory/invasive tumor cells endowed with immunomodulator function". PLOS ONE. 6 (4): e18784. doi:10.1371/journal.pone.0018784. PMC 3078142. PMID 21526207.

[Mahmood2002-3] Mahmood, M. N.; Lee, M. W.; Linden, M. D.; Nathanson, S. D.; Hornyak, T. J.; Zarbo, R. J. (2002). "Diagnostic Value of HMB-45 and Anti-Melan a Staining of Sentinel Lymph Nodes with Isolated Positive Cells". Modern Pathology. 15 (12): 1288–1293. doi:10.1097/01.MP.0000037313.33138.DF. PMID 12481009.

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