Zymolyase
Zymolyase (also known as lyticase) is an enzyme mixture used to degrade the cell wall of yeast and form spheroplasts.[1][2] Essential activities of zymolyase include β-1,3-glucan laminaripentao-hydrolase activity and β-1,3-glucanase activity. A common source of zymolyase is the Actinobacteria Arthrobacter luteus.[1] Commercial sources of zymolyase may have some residual protease activity.
Fungi susceptible to zymolyase can be found in the order Saccharomycetales.
Saccharomycetales genera that zymolyase is known to have activity against include:[3]
Eremothecium, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloekera, Kluyveromyces, Lipomyces, Metschikowia, Pichia, Pullularia, Saccharomyces, Saccharomycopsis, Schizosaccahromyces, Torulopsis
References
- ^ a b Calvert, C.M.; Sanders, D. (1995). "Inositol trisphosphate-dependent and -independent Ca2+ mobilization pathways at the vacuolar membrane of Candida albicans" (PDF). The Journal of Biological Chemistry. 270 (13): 7272–80. doi:10.1074/jbc.270.13.7272. PMID 7706267.
- ^ "Lyticase from Arthrobacter luteus". Millipore-Sigma. Retrieved 2019-03-22.
- ^ "Zymolyase". Zymo Research. Retrieved 2019-03-22.