HaCaT

HaCaT is a spontaneously transformed aneuploid immortal keratinocyte cell line from adult human skin,^[1]^[2] widely used in scientific research.^[3] HaCaT cells are utilized for their high capacity to differentiate and proliferate in vitro.^[4] Their use in research allows for the characterization of human keratinocyte using a model that is reproducible and addresses issues such as short culture lifespan and variations between cell lines that would otherwise be encountered. These cells have allowed the characterization of several processes, such as their utilization as a model system for vitamin D₃ metabolism in the skin.^[5]

References

^ Boukamp, Petra; Petrussevska, Rule T.; Breitkreutz, Dirk; Hornung, Jiirgen; Markham, Alex; Fusenig, Norbert E. (1 March 1988). "Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line". The Journal of Cell Biology. 106 (3): 761–771. doi:10.1083/jcb.106.3.761. PMC 2115116. PMID 2450098.
^ Ölschläger, Veronika; Schrader, Andreas; Hockertz, Stefan (13 December 2011). "Comparison of Primary Human Fibroblasts and Keratinocytes with Immortalized Cell Lines Regarding their Sensitivity to Sodium Dodecyl Sulfate in a Neutral Red Uptake Cytotoxicity Assay". Arzneimittelforschung. 59 (3): 146–152. doi:10.1055/s-0031-1296378. PMID 19402346. S2CID 20840726.
^ Schoop, Veronika M.; Fusenig, Norbert E.; Mirancea, Nicolae (March 1999). "Epidermal Organization and Differentiation of HaCaT Keratinocytes in Organotypic Coculture with Human Dermal Fibroblasts". Journal of Investigative Dermatology. 112 (3): 343–353. doi:10.1046/j.1523-1747.1999.00524.x. PMID 10084313.
^ Schürer, N; Köhne, A; Schliep, V; Barlag, K; Goerz, G (Aug 1993). "Lipid composition and synthesis of HaCaT cells, an immortalized human keratinocyte line, in comparison with normal human adult keratinocytes". Experimental Dermatology. 2 (4): 179–85. doi:10.1111/j.1600-0625.1993.tb00030.x. PMID 8162337. S2CID 20709063.
^ Lehmann, Bodo (1 January 1997). "HaCaT Cell Line as a Model System for Vitamin D3 Metabolism in Human Skin". Journal of Investigative Dermatology. 108 (1): 78–82. doi:10.1111/1523-1747.ep12285640. PMID 8980292.

External links

[1] Boukamp, Petra; Petrussevska, Rule T.; Breitkreutz, Dirk; Hornung, Jiirgen; Markham, Alex; Fusenig, Norbert E. (1 March 1988). "Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line". The Journal of Cell Biology. 106 (3): 761–771. doi:10.1083/jcb.106.3.761. PMC 2115116. PMID 2450098.

[2] Ölschläger, Veronika; Schrader, Andreas; Hockertz, Stefan (13 December 2011). "Comparison of Primary Human Fibroblasts and Keratinocytes with Immortalized Cell Lines Regarding their Sensitivity to Sodium Dodecyl Sulfate in a Neutral Red Uptake Cytotoxicity Assay". Arzneimittelforschung. 59 (3): 146–152. doi:10.1055/s-0031-1296378. PMID 19402346. S2CID 20840726.

[3] Schoop, Veronika M.; Fusenig, Norbert E.; Mirancea, Nicolae (March 1999). "Epidermal Organization and Differentiation of HaCaT Keratinocytes in Organotypic Coculture with Human Dermal Fibroblasts". Journal of Investigative Dermatology. 112 (3): 343–353. doi:10.1046/j.1523-1747.1999.00524.x. PMID 10084313.

[4] Schürer, N; Köhne, A; Schliep, V; Barlag, K; Goerz, G (Aug 1993). "Lipid composition and synthesis of HaCaT cells, an immortalized human keratinocyte line, in comparison with normal human adult keratinocytes". Experimental Dermatology. 2 (4): 179–85. doi:10.1111/j.1600-0625.1993.tb00030.x. PMID 8162337. S2CID 20709063.

[5] Lehmann, Bodo (1 January 1997). "HaCaT Cell Line as a Model System for Vitamin D3 Metabolism in Human Skin". Journal of Investigative Dermatology. 108 (1): 78–82. doi:10.1111/1523-1747.ep12285640. PMID 8980292.

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