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Smash and Grab (biology)

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This is an old revision of this page, as edited by Clinamental (talk | contribs) at 23:01, 29 March 2018 ('southern blot' [lower case] ---> 'Southern blot' [upper case]; named after the inventor Sir Edwin Southern.). The present address (URL) is a permanent link to this revision, which may differ significantly from the current revision.

This article is about a technique in molecular biology. For other uses, see Smash and grab (disambiguation).

Smash and Grab is the name given to a technique developed by Charles S. Hoffman and Fred Winston used in molecular biology to rescue plasmids from yeast transformants into Escherichia coli, also known as E. coli, in order to amplify and purify them.[1] In addition, it can be used to prepare yeast genomic DNA (and DNA from tissue samples) for Southern blot analyses or polymerase chain reaction (PCR).

References

  1. ^ C.S. Hoffman; F. Winston (1987). "A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli". Gene. 57 (2–3): 267–72. doi:10.1016/0378-1119(87)90131-4. PMID 3319781.
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