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pBLU

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A map of pBLU

pBLU is a commercially produced bacterial plasmid that contains genes for ampicillin resistance (beta lactamase and beta galactosidase). It is often used in conjunction with an ampicillin-susceptible E. coli strain to teach students about transformation of eubacteria.[1] It is 5,437 base pairs long. There is a multiple cloning site in the lacZ gene.

References

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  1. ^ "BIOTECHNOLOGY: BACTERIAL TRANSFORMATION" (PDF). CollegeBoard. Retrieved 29 October 2017.