Cytochemistry

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Transmission electron micrograph of a chondrocyte, stained for calcium, showing its nucleus (N) and mitochondria (M).

Cytochemistry is the branch of cell biology dealing with the detection of cell constituents by means of biochemical analysis and visualization techniques. The term is also used to describe a process of identification of the biochemical content of cells. Cytochemistry is a science of localizing chemical components of cells and cell organelles on thin histological sections by using several techniques like enzyme localization, micro-incineration, micro-spectrophotometry, radioautography, cryo-electron microscopy, X-ray microanalysis by energy-dispersive X-ray spectroscopy, immunohistochemistry and cytochemistry, etc.[1]

Footnotes

  1. ^ Nagata, T (2001). "Special cytochemistry in cell biology". International Review of Cytology. 211: 33–151. PMID 11597006.

References

  • Brighton, Carl T. and Robert M. Hunt (1974). "Mitochondrial calcium and its role in calcification". Clinical Orthopaedics and Related Research 100: 406-416.
  • Brighton, Carl T. and Robert M. Hunt (1978). "The role of mitochondria in growth plate calcification as demonstrated in a rachitic model". Journal of Bone and Joint Surgery, 60-A: 630-639.


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