Plaque hybridization

Plaque hybridization is a technique used in Molecular biology for the identification of recombinant phages.^[1] The procedure can also be used for the detection of differentially represented repetitive DNA. The technique (similar to colony hybridization) involves hybridizing isolated phage DNA to a label probe for the gene of study. This is followed by autoradiography to detect the position of the label.^[2] The plaque hybridization procedure has some advantages over colony hybridization due to the smaller and well defined area of the filter to which the DNA binds.^[3]

References

^ Dale, J. W.; Greenaway, P. J. (1985). Nucleic Acids. Methods in Molecular Biology. Vol. 2. pp. 285–8. doi:10.1385/0-89603-064-4:285. ISBN 0-89603-064-4. PMID 21374207.
^ Meister, G. A.; Lansman, R. A.; Grigliatti, T. A. (1995). "Simple plaque hybridization method for the detection of differentially represented repetitive DNA". BioTechniques. 18 (2): 250–5. PMID 7727126.
^ "Nylon Membranes for Colony and Plaque Hybridization". Life science. Retrieved June 4, 2015.

[pmid21374207-1] Dale, J. W.; Greenaway, P. J. (1985). Nucleic Acids. Methods in Molecular Biology. Vol. 2. pp. 285–8. doi:10.1385/0-89603-064-4:285. ISBN 0-89603-064-4. PMID 21374207.

[pmid7727126-2] Meister, G. A.; Lansman, R. A.; Grigliatti, T. A. (1995). "Simple plaque hybridization method for the detection of differentially represented repetitive DNA". BioTechniques. 18 (2): 250–5. PMID 7727126.

[3] "Nylon Membranes for Colony and Plaque Hybridization". Life science. Retrieved June 4, 2015.

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