Folin's reagent

Folin's reagent
Names
	IUPAC name Sodium 3,4-dioxo-3,4-dihydronaphthalene-1-sulfonate
Identifiers
CAS Number	521-24-4 ;
3D model (JSmol)	Interactive image;
ChemSpider	10190;
ECHA InfoCard	100.007.555
EC Number	208-308-9;
PubChem CID	10636;
UNII	T7185HP5SH ;
CompTox Dashboard (EPA)	DTXSID8060167 ;
	InChI InChI=1S/C10H6O5S.Na/c11-8-5-9(16(13,14)15)6-3-1-2-4-7(6)10(8)12;/h1-5H,(H,13,14,15);/q;+1/p-1 Key: UBLXEEBHYISRFM-UHFFFAOYSA-M; InChI=1/C10H6O5S.Na/c11-8-5-9(16(13,14)15)6-3-1-2-4-7(6)10(8)12;/h1-5H,(H,13,14,15);/q;+1/p-1 Key: UBLXEEBHYISRFM-REWHXWOFAQ;
	SMILES C1=CC=C2C(=C1)C(=CC(=O)C2=O)S(=O)(=O)[O-].[Na+];
Properties
Chemical formula	C10H5NaO5S
Molar mass	260.20 g/mol
Hazards
Safety data sheet (SDS)	Oxford MSDS
	Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). verify (what is ?) Infobox references

Folin's reagent or sodium 1,2-naphthoquinone-4-sulfonate is a chemical reagent used as a derivatizing agent to measure levels of amines and amino acids.^[1] The reagent reacts with them in alkaline solution to produce a fluorescent material that can be easily detected.^[2]

This should not be confused with Folin-Ciocalteu reagent, that is used to detect phenolic compounds.

The Folin reagent can be used with an acidic secondary reagent to distinguish MDMA and related compounds from PMMA and related compounds.^[3]^{[failed verification]}

Substance	Reaction
MDMA	Pink^[3]
MDE	No Reaction^[3]
MDA	Yellow^[3]
BZP	Orange to red^[3]
TFMPP	Pinkish red^[3]
Methylone	No reaction^[3]
Methamphetamine	Pink^[3]
Amphetamine	Light orange^[3]
PMA	Light Yellow^[3]
PMMA	Light orange^[3]
Ketamine	No reaction^[3]
Mescaline	No reaction^[3]
Cocaine	No reaction^[3]
Aspirin	No reaction^[3]
Sugar	No reaction^[3]

References

^ Saurina J, Hernández-Cassou S (1994). "Determination of amino acids by ion-pair liquid chromatography with post-column derivatization using 1,2-naphthoquinone-4-sulfonate". Journal of Chromatography A. 676 (2): 311–9. doi:10.1016/0021-9673(94)80431-1. PMID 7921184.
^ Kobayashi Y, Kubo H, Kinoshita T (1987). "Fluorometric determination of guanidino compounds by new postcolumn derivatization system using reversed-phase ion-pair high-performance liquid chromatography". Anal. Biochem. 160 (2): 392–8. doi:10.1016/0003-2697(87)90066-2. PMID 3578768.
^ ^a ^b ^c ^d ^e ^f ^g ^h ⁱ ^j ^k ^l ^m ⁿ ^o ^p "Folin Reagent Testing Kit". Dancesafe. June 2015. Archived from the original on 11 September 2015. Retrieved 8 August 2015.

[1] Saurina J, Hernández-Cassou S (1994). "Determination of amino acids by ion-pair liquid chromatography with post-column derivatization using 1,2-naphthoquinone-4-sulfonate". Journal of Chromatography A. 676 (2): 311–9. doi:10.1016/0021-9673(94)80431-1. PMID 7921184.

[2] Kobayashi Y, Kubo H, Kinoshita T (1987). "Fluorometric determination of guanidino compounds by new postcolumn derivatization system using reversed-phase ion-pair high-performance liquid chromatography". Anal. Biochem. 160 (2): 392–8. doi:10.1016/0003-2697(87)90066-2. PMID 3578768.

[Dancesafe_Folin-3] ^ ^a ^b ^c ^d ^e ^f ^g ^h ⁱ ^j ^k ^l ^m ⁿ ^o ^p "Folin Reagent Testing Kit". Dancesafe. June 2015. Archived from the original on 11 September 2015. Retrieved 8 August 2015.

[1]

[2]

[3]

See also

References